肿瘤坏死因子相关凋亡诱导配体受体在人肝细胞肝癌的原发灶及其门脉癌栓中的表达及意义  

Expression and significance of TNF-related apoptosis inducing ligand receptors in the primary lesions and portal vein tumor thrombi of the human hepatocellular carcinoma

作　　者：梅洪亮[1] 张志伟[1] 沈先锋[1] 张贯启[1] 陈孝平[1] 吴在德[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030

出　　处：《中华实验外科杂志》2010年第2期164-166,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）受体DR4、DR5、DcR1、DcR2在人肝细胞肝癌的原发灶及其门脉癌栓中的表达及意义.方法 采用实时荧光定量逆转录-聚合酶链反应（RT-PCR）法,检测20例人肝细胞肝癌的原发灶及其门脉癌栓和20例未发生转移的原发性肝癌组织中TRAIL受体mRNA的表达水平.结果 死亡受体DR4、DB5在无转移的肝癌组织分别为（3.59±0.87）、（1.98±0.54）,伴有门脉癌栓的肝癌原发灶组织（分别设定为1）及其门脉癌栓组织中的表达量分别为（0.62±0.28）、（0.31±0.12）,呈递减趋势（P〈0.05）.诱捕受体DcR1、DcR2在伴有门脉癌栓的肝癌组织中的表达量分别为（0.29±0.04）、（0.54±0.08）,显著低于无转移的肝癌组织（分别设定为1）（P〈0.05）.而在伴发PVTT的HCC中,门脉癌栓组织与其肝癌原发灶组织中DcR的表达量差异无统计学意义（P〉0.05）.DR的表达水平与肿瘤的分化程度（r=0.461,P〈0.05）及门静脉浸润情况（r=0.587,P〈0.05）呈显著正相关.DR的表达水平与肿瘤的大小及血清甲胎蛋白（AFP）浓度无明显相关（P〉0.05）.结论 TRAIL死亡受体DR的表达下调可能与肝癌的恶性进展密切相关.TRAIL 途径诱导凋亡在肝癌转移过程中可能起到重要的作用.Objective To investigate the expression and significance of TNF-related apoptosis inducing ligand （TRAIL） receptors in the primary lesions and portal vein tumor thrombi （PVTT） of the human hepatocellular carcinoma （HCC）. Methods The expression of TRAIL receptors mRNA was detected by real-time fluorescence quantitative RT-PCR in the primary lesions and portal vein tuner thrombi of 20 samples of HCC, and in the tissues of 20 samples of primary hepatic carcinoma without metastasis. Results The expression of death receptors （DR4, DR5） was decreased from the primary hepatic carcinoma without metastasis [（3.59±0.87）, （1.98±0.54）, respectively], the primary lesions of HCC with PVTY （set it as 1 respectively） to the PVTr [（0.62±0.28）, （0.31±0.12）, respectively]（P〈0.05）. The expression of decoy receptors （DcR1, DcR2） in the HCC with PVTT [（0.29±0.04）, （0.54±0.06）, respectively]was significantly lower than that in the primary hepatic carcinoma without metastasis （set it as 1 respectively） （P〈0.05）. However, the expression of decoy receptors had no significant difference between the primary lesions of HCC with PVTT （P〉0.05）. The expression level of decoy receptors was had significantly positive correlation with the tumor differentiation （r=0.461, P〈0.05） and portal vein invasion （r= 0.587,P〈0.05）. However, the expression level had no significant correlation with the tumor size and serum concentration of a-fetoprotein （AFP, P〉0.05 ）. Conclusion Lower expression of TRAIL death re-ceptors may be closely correlated to the malignant progression of HCC. TRAIL-induced apoptosis may play an important role in the metastatic process of HCC.

关 键 词：癌 肝细胞 TRAIL受体 转移 脱噬作用 

分 类 号：R735.7[医药卫生—肿瘤]