胶质瘤间质血管增生和细胞增殖与环氧化酶-2基因的关系  

Relationship of cyclooxygenase with vascularization and proliferation in human glioma

作　　者：吴涛[1] 刘胜[1] 王诚[1] 高玉红[1] 

机构地区：[1]北京大学深圳医院神经外科,深圳518036

出　　处：《中华实验外科杂志》2010年第2期221-223,共3页Chinese Journal of Experimental Surgery

基　　金：深圳市科技计划资助项目（20090359）

摘　　要：目的 探讨环氧化酶-2（COX-2）基因与胶质瘤间质血管增生和细胞增殖的关系.方法 用原位杂交方法 检测胶质瘤组织中COX-2 mRNA的表达;用免疫组织化学检测同等标本中细胞增殖指数（Ki-67LI）及微血管密度（MVD）的表达;并将COX-2 mRNA与Ki-67、CD34作相关分析.结果 胶质瘤中COX-2 mRNA表达阳性率为62.69%,对照脑组织中无阳性表达.COX-2 mRNA阳性着色定位于微血管周围,呈棕黄色园环状表现.C0x-2 mRNA表达阳性率在低度恶性胶质瘤（Ⅰ、Ⅱ级）中为37.14%（14/35）,而在高度恶性胶质瘤（Ⅲ、Ⅳ级）中为87.50%.在低度、高度恶性胶质瘤中MVD分别为12.54±3.11和20.31±5.49;其Ki-67LI分别为12.29±5.12和23.31±7.14.COX-2 mRNA表达为阴性和阳性胶质瘤中其MVD分别为16.90±6.48和9.46±2.15,其Ki-67LI分别为18.73±8.87和11.10±2.93.结论 COX-2 mRNA在胶质瘤组织中的表达与胶质瘤间质血管增生和细胞增殖密切相关;MVD和Ki-67U在COX-2 mRNA表达为阴性和阳性组织中比较差异有统计学意义.Objective Investigate the relationship of cyclooxygenase （COX-2） with vascularization and proliferation in human glioma. Methods The expression of COX-2 mRNA was detected in 67 cases of ghoma and 8 cases of normal brain specimens by using in situ hibridization method. Ki-67 and CD34 protein expression was assayed in 67 cases of ghoma and 8 cases of normal brain specimens by immunohistochemistry to investigate the vascularization and proliferation. Results The expression of COX mRNA was detected in glioma tissue and not in normal brain specimens. The expression of Ki-67 and CD34 was significantly higher in COX-2 positive specimens than in COX-2 negative specimens, and the expression of Ki-67 and CD34 was significantly higher in high-grade ghoma than low-grade ghoma and normal brain specimens. Conclusion Glioma cell proliferation and vascularization strongly connects with COX-2 gene. The study suggests that COX-2 gene may play an important role in human glioma cell proliferation and vascularization.

关 键 词：胶质瘤 血管增生 环氧化酶-2 

分 类 号：R739.41[医药卫生—肿瘤]