CCL5对MCF-7中细胞增殖能力及CD44＋／CD24-亚群比例的影响  

Effect of CCL5 on cell proliferation and the proportion of CD44＋ ＋/CD24-subpopulation of breast cancer cell line

作　　者：魏文[1] 张懿敏[1] 孙圣荣[1] 王卫星[1] 

机构地区：[1]武汉大学人民医院乳腺甲状腺外科,430060

出　　处：《中华实验外科杂志》2010年第3期348-349,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省卫生厅资助项目（JS3A14）

摘　　要：目的观察外源性趋化因子5（CCL5）对人乳腺癌MCF-7细胞增殖及CD44＋／CD24-亚群比例的影响，探讨其在乳腺癌进展过程中的作用。方法噻唑蓝（MTT）比色法检测浓度为10、50、100、500μg／L的外源性rhCCL5（recombinant human CCL5）作用0、24、48h后MCF-7细胞增殖水平的变化，流式细胞仪检测CD44＋／CD24-亚群比例的变化。结果rhCCL5作用48h后MCF-7细胞增殖水平高于对照组（P〈0．05），并呈浓度依赖趋势；且高于作用24、0h后细胞的增殖水平（P〈0．05）。50、500μg／LrhCCL5处理MCF-7细胞后升高了细胞株中CD44＋／CD24-亚群的比例，分别为对照组的5．68和6．05倍（P〈0．05）。结论外源性CCL5可以明显升高MCF-7细胞的增殖水平以及CD44＋／CD24-亚群的比例，这种作用可能是其诱导乳腺癌进展的机制之一。Objective To observe the effects of CC chemokine ligand 5 （CCL5） on cell proliferation and the proportion of CD44＋/CD24- subpopulation in breast cancer cell line MCF-7, and to investigate the role of CCL-5 in the progression of breast cancer. Methods Effects of recombinant human CCL-5 （rhCCL-5） on cell proliferation of MCF-7 ceils were detected by MTT assay, and the percentage of the CD44＋/CD24- subpopulation in breast cancer cell line MCF-7 was analyzed by flow cytometry. Results When MCF-7 cells were treated with rhCCL5 .（ 10, 50, 100,500 μg/L） for 48 h, the viability of cells was increased in a concentration-dependent manner （ P 〈 0.05 ）. The viability of MCF-7 cells treated with 100 μg/L rhCCL5 for 48 h was higher than that for 24 and 0 h （ P 〈 0. 05 ）. The proportion of CD44 ＋/CD24 -subpopulation in MCF-7 cell line was elevated to 5.68-fold of the control cells, when treated with 50 μg/L rhCCI-5 （P 〈 0.05 ）, and to 6.05-fold, when treated with 500 μg/L rhCCL5 （P 〈 0.05）. Conclusion CCL5 could elevate cell proliferation capacity and the proportion of CD44＋/CD24 - subpopulation of MCF-7 cells, which might be one of the possible mechanisms of CCL5 stimulating the progression of breast cancer.

关 键 词：乳腺癌 趋化因子5 细胞增殖 CD44 

分 类 号：R737.9[医药卫生—肿瘤]