经皮瘤内注射艾迪注射液治疗兔移植性VX2肝肿瘤  

Treatment of the implanted VX2 liver tumor in rabbits with percutaneous intratumor AiDi injection

作　　者：陈翼[1] 黄永火[1] 康小兵 

机构地区：[1]重庆医科大学附属第一医院放射科,重庆400016 [2]重庆市江北区第一人民医院放射科,重庆400020

出　　处：《中国介入影像与治疗学》2010年第2期192-195,共4页Chinese Journal of Interventional Imaging and Therapy

摘　　要：目的探讨超声引导下复方中药艾迪注射液瘤内注射治疗兔移植性VX2肝肿瘤的作用效果及机制。方法建立新西兰大白兔移植性肝VX2肿瘤模型28只,随机分为3组,即艾迪注射液组(12只)、无水乙醇组(PEI组,8只)、生理盐水组(8只)。全部动物在接种2周后每隔3天向肿瘤内注入治疗药物,注射4次后处死动物。于术前术后兔耳缘静脉抽血行血生化检查,同时行64排螺旋CT扫描测量并计算肿瘤体积、肿瘤增长率及肿瘤坏死率,以及进行组织细胞学与细胞超微结构的观察。结果各实验组病灶均进行性增大,但艾迪注射液组肿瘤增长率及坏死率最小,与生理盐水组比较差异均有统计学意义(P<0.05),与无水乙醇组比较差异无统计学意义;TUNEL法检测显示艾迪注射液组凋亡指数明显高于无水乙醇组及生理盐水组(P<0.05);艾迪注射液组肝肾功能及白细胞计数治疗前后均无明显差异,组内及组间比较差异无统计学意义(P>0.05)。结论艾迪注射液可诱导肿瘤细胞凋亡,对肿瘤细胞具有细胞毒性作用。Objective To explore the principle,effect and method of percutaneous AiDi injection for treatment of implanted VX2 liver tumor with ultrasound guidance.Methods Twenty-eight New Zealand white rabbits with implanted VX2 liver tumors were randomly divided into 3 groups： AiDi injection group（n=12）,percutaneous ethanol injection（PEI） group（n=8）,and physiological saline group（n=8）.Two weeks after transplantation,the drugs were injected into tumors of all the rabbits once every 3 days.After 4 times injection,the rabbits were killed.The tumors were measured with 64-slice CT,while the rate of tumor necrosis,the volume of tumor and the growth rate of the tumor were calculated.Liver and kidney function tests（AST,ALT,ALP and BUN） as well as white blood cell counts were assessed before and after treatment,and CT examination was performed at the same time.The morphological and the ultramicrostructural changes were also investigated under light microscope or electron microscope.Results Tumors in all groups kept growing after the procedure.Among all 3 groups,tumor growing rate for AiDi injection group was the lowest and the necrotic rate was the highest（P〈0.05）,while no significant difference was found between AiDi injection group and PEI group（P〉0.05）.Apoptotic index in AiDi injection group was significantly higher than that in PEI group and physiological saline group（P〈0.05）.There was no evident change of liver and kidney function before and after treatment in AiDi injection group and PEI group（P〉0.05）.Conclusion AiDi injection can induce apoptosis of liver VX2 tumor cells.Percutaneous AiDi injection therapy can obviously inhibit and destroy liver VX2 tumor cells.

关 键 词：兔 肝脏 VX2 模型 动物 艾迪注射液 

分 类 号：R735.7[医药卫生—肿瘤]