从适应低温的细菌中提取的低温酶(英)  

Cold-Active Enzymes from Cold-Adapted Bacteria

作　　者：守田安宅 胡成香 陆剑英 

机构地区：[1]山东佳丽日化总公司技术开发中心

出　　处：《日用化学品科学》1998年第6期27-29,共3页Detergent & Cosmetics

摘　　要：从覆盖有积雪的土壤中的大麻哈鱼肠和螃蟹肠中分离出了新的细菌苗种，并对它们分泌出的淀粉酶、脂肪酶和蛋白酶的性能进行了研究。发现了一种淀粉酶、一种脂肪酶和三种蛋白酶，它们的最佳活性温度明显地朝低温方向移动，而且活化能有所降低。这些酶在高于最低温度（即３０℃～４０℃）时迅速失活。结果表明，这些酶具有低温活性。低温活性最好的蛋白酶生产者从大麻哈鱼肠中分离出来的菌种通过１６Ｓｒ核糖核酸分析已验明为黄杆菌属，这种细菌的最适生长温度为２０℃。The properties of amylase,lipase and protease,excreted by newly isolated bacteria from snow-covered soil,salmon intestine and crab intestine,have been investigated.One amylase,one lipase,and three proteases have been characterized by shifts in their apparent optimal activities toword low temperatures and by reductions in their activation energy values.The discovered enzymes were rapidly inactivated at temperatures above the optimum(30℃ to 40℃).These results suggest that the enzymes are cold-active.The best cold-active protease producer,isolated from salmon intestine,has been identified as flavobacterium balustinum by the analysis of 16SrRNA.The optimal growth temperature of this bacterium was 20℃,but a higher amount of protease activity was present at 10℃.

关 键 词：活化能 低温活性 低温酶 细菌 酶提取 

分 类 号：Q936[生物学—微生物学]