重组人生长激素对荷人胃癌细胞SGC-7901裸鼠移植瘤生长及VEGF表达的影响  

Recombinant human growth hormone promotes tumor growth and VEGF expression in subcutaneous xenografts derived from human gastric carcinoma SGC-7901 cells in nude mice

作　　者：程璐[1] 苏翔宇[1] 林岩[2] 李苏宜[1] 

机构地区：[1]东南大学附属中大医院肿瘤科,江苏省南京市210009 [2]南京医科大学第二附属医院肿瘤科,江苏省南京市210011

出　　处：《世界华人消化杂志》2010年第6期536-541,共6页World Chinese Journal of Digestology

基　　金：南京市医学科技发展项目基金资助项目;No.YKK07097~~

摘　　要：目的:探讨重组人生长激素(rhGH)对荷人胃癌细胞株SGC-7901裸鼠移植瘤生长及VEGF表达的影响.方法:免疫细胞化学染色法鉴定SGC-7901细胞株GHR表达状态,30只接种皮下移植瘤的裸鼠随机分为:对照组(生理盐水0.2mL/d),低剂量rhGH组[0.5U/(kg·d)],高剂量rhGH组[2.5U/(kg·d)],连续给药14d,观察并记录裸鼠体质量和肿瘤体积,酶联免疫吸附法测定血清VEGF含量,免疫组织化学法检测胃癌组织中VEGF蛋白表达,RT-PCR检测VEGFmRNA表达.结果:SGC-7901细胞株GHR呈强阳性表达.自rhGH给药第3天起,rhGH给药组与对照组肿瘤体积相差悬殊(P<0.05),且高剂量rhGH比低剂量rhGH促肿瘤生长效应更加明显(P<0.05),3组间裸鼠体质量差别不明显(P>0.05).裸鼠血清VEGF含量,与对照组和低剂量rhGH组相比,高剂量rhGH组血清中VEGF水平明显升高,差别具有统计学意义(252.94ng/L±15.32ng/Lvs49.94ng/L±5.73ng/L,167.60ng/L±9.54ng/L,均P<0.05).肿瘤组织VEGF蛋白的表达,对照组VEGF表达呈中度阳性;低剂量rhGH组和高剂量rhGH组VEGF表达量高,呈强阳性.肿瘤组织VEGFmRNA表达水平,高剂量rhGH组VEGF相对表达量明显高于对照组和低剂量rhGH组,差别具有统计学意义(0.647±0.0447vs0.323±0.0258,0.412±0.0351,均P<0.05).结论:rhGH能促进GHR阳性表达的SGC-7901移植瘤生长,并促进VEGF表达.AIM： To investigate the effects of recombinant human growth hormone （rhGH） on tumor growth and VEGF expression in subcutaneous xenografts derived from human gastric carcinoma SGC-7901 cells in nude mice. METHODS： The expression of growth hormone receptor （GHR） in human gastric carcinoma cell line SGC-7901 was detected by immuno-cytochemistry. Thirty nude mice bearing sub-cutaneous xenografts derived from carcinoma SGC-7901 cells were randomly divided into three groups： control group, low-dose rhGH group and high-dose rhGH group. The low-and high-dose rhGH groups were injected with rhGH at doses of 0.5 and 2.5 U/（kg？d） once a day for two weeks, respectively, while the control group was injected with equal volumes of normal saline for the same duration. The changes in body weight and tumor volume were recorded. The content of serum VEGF in peripheral blood was ana-lyzed by enzyme-linked immunosorbent assay （ELISA）. The expression of VEGF mRNA and protein in tumor tissue was detected by re-verse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry, respec-tively. RESULTS： GHR is highly expressed in SGC-7901 cells. After treatment with rhGH for three days, the tumor volume was significantly larger in the two rhGH groups than in the con-trol group （both P 0.05）. High-dose rhGH revealed stronger tumor growth-promoting effect than low-dose one （P 0.05）. No significant difference was found in the body weight of nude mice among the three groups （all P 0.05）. The content of serum VEGF was elevated more obviously in the high-dose rhGH group than in the low-dose rhGH group and the control group. （252.94 ng/L ± 15.32 ng/L vs 167.60 ng/L ± 9.54 ng/L and 49.94 ng/L ± 5.73 ng/L, respectively; both P 0.05）. The expression level of VEGF protein in tumor tissue was signifi cantly higher in the two rhGH groups than in the control group. The relative expression level of VEGF mRNA was much higher in the high-dose rhGH group than in the low-dose rhGH group and the co

关 键 词：重组人生长激素 生长激素受体 血管内皮生长因子 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]