海洋胶原肽对高胰岛素血症模型大鼠糖脂代谢的影响  

Effects of marine collagen peptides on blood glucose and lipid metabolism in hyperinsulinemic rats

作　　者：王军波[1] 张召锋[1] 裴新荣[2] 杨睿悦[3] 李勇[1] 

机构地区：[1]北京大学公共卫生学院营养与食品卫生学系,北京100191 [2]首都医科大学食品药品安全评价中心 [3]卫生部北京医院/卫生部北京老年医学研究所卫生部老年医学重点实验室

出　　处：《卫生研究》2010年第2期143-146,共4页Journal of Hygiene Research

基　　金：国家"十一五"科技支撑计划项目(No.2006BAD27B01)

摘　　要：目的研究海洋胶原肽(MCPs)对高胰岛素血症模型大鼠糖脂代谢的影响。方法雄性SD大鼠以高脂饲料喂养4个月建立高胰岛素血症模型,按照体重和空腹胰岛素水平分为高胰岛素血症对照组(HIC)、0.225、0.45和1.35g/kgbw海洋胶原肽干预组,另选以基础饲料喂养4个月的健康雄性SD大鼠作为空白对照组。MCPs干预组大鼠以灌胃方式给予不同剂量的MCPs,HIC和空白对照组给予等体积蒸馏水灌胃。实验期间HIC及MCPs各组继续喂饲高脂饲料,空白对照组喂饲基础饲料,实验周期8周。于实验结束时进行口服糖耐量实验,检测空腹血糖、胰岛素、血脂水平、抗氧化酶活性及血清丙二醛含量,并观察胰岛β细胞的超微结构改变。结果实验结束时,与HIC组比较,各剂量MCPs组大鼠空腹胰岛素、总胆固醇和甘油三酯水平明显下降;0.225和1.35g/kgbw MCPs组大鼠空腹血糖水平明显降低,0.45和1.35g/kgbw MCPs组大鼠OGTT0.5h、2h血糖水平和血糖曲线下面积明显降低;各剂量MCPs组大鼠血清超氧化物歧化酶活性明显升高,1.35g/kgbw MCPs组大鼠血清谷胱甘肽过氧化物酶活性明显提高,而血清丙二醛含量明显减少,同时胰岛β细胞的超微结构得到明显改善。结论MCPs对高脂饲料诱导的高胰岛素血症大鼠糖脂代谢紊乱具有明显的改善作用。Objective To observe the effects of marine collagen peptides ( MCPs) on blood glucose and lipid metabolism in hyperinsulinemic rats. Methods Male SD rats fed with high fat diet for 4 months were used to establish a hyperinsulinemic model. Thirty two hyperinsulinemic rats were divided into four groups according to body weight and serum insulin level,namely hyperinsulinemic control ( HIC) group and 3 Marine Collagen Peptides treated groups with 8 rats in each group. Another 8 healthy male SD rats fed with basic diet were used as negative control (NC). Rats in MCPs groups were treated by intragastric way with MCPs at the dose of 0. 225g /kg bw, 0. 45g /kg bw and 1. 35g /kg bw respectively for 8 weeks,while rats in HIC and NC groups were treated with tap water in the same manner. During the test period,rats in HIC and MCPs groups were fed with high fat diet sequentially,while rats in NC group were fed with basic diet. Fasting serum glucose ( GLU),insulin ( INS) and lipids were assayed respectively before and after MCPs treatment. Oral glucose tolerance test,antioxidase activity, serum malondialdehyde ( MDA) levels and the ultrastructure of islet β cell were carried out at the end of the experiment. Results After 8 weeks of treatment,compared with HIC group,fasting INS,TC and TG of all three MCPs-treated groups decreased obviously,while fasting GLU level lowered significantly in 0. 225g /kg bw and 1. 35g /kg bw MCPs groups. Moreover,GLU level at 0. 5h,2h and the area under blood sugar curve in OGTT were evidently lower in 0. 45g /kg bw and 1. 35g /kg bw MCPs groups. Besides,serum SOD activity increased in all MCPs groups,and serum GSH-Px activity elevated obviously in 1. 35g /kg bw MCPs group,while serum MDA reduced.Finally,the ultrastructural impairment of islet resulted from high fat diet was improved evidently by 1. 35g /kg bw MCPs. Conclusion MCPs was effective in improving glucose and lipid metabolism in hyperinsulinemic rats induced by high fat diet.

关 键 词：海洋胶原肽 高胰岛素血症 糖脂代谢 

分 类 号：R587.1[医药卫生—内分泌] Q591.1[医药卫生—内科学]