桑树根际硅酸盐细菌的分离鉴定及解钾能力测定  

Isolation and Identification of Mulberry Rhizospheric Silicate Bacteria and Determination of Their Potassium-releasing Activities

作　　者：吴凡[1,2] 刘训理[1] 张楠[1] 张莎莎[1] 国辉[1] 张本峰[1] 仇念全[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]湖北省农业科学院经济作物研究所,湖北武汉430064

出　　处：《蚕业科学》2010年第2期323-329,共7页ACTA SERICOLOGICA SINICA

基　　金：山东省科技攻关计划项目(No.02201010102)

摘　　要：利用选择性培养基,从桑树根际土样中分离获得29个具有解钾能力的细菌分离株,经rep-PCR DNA指纹分析得到24株硅酸盐细菌。通过解钾能力测定,筛选出FK2、FK3、FK8、FK11、FK4′、FK23和PK187个具有较强解钾能力的菌株,其中FK2菌株的解钾能力最强,有效态钾增长41.79%。对这7株具有较强解钾能力的细菌进行菌落形态特征观察及16S rDNA序列测定和同源性分析:FK2菌株为假单胞菌属(Pseudomonas sp.),FK3和FK23菌株为中华根瘤菌属(Sinorhizobium sp.),FK11和FK8菌株为根瘤菌属(Rhizobiumsp.),FK4′菌株为中慢生根瘤菌属(Mesorhizobium sp.),PK18菌株为屈挠杆菌属(Flexibacter sp.)。29 bacterial isolates that had potassium-releasing activities were obtained from mulberry rhizospheric soil through cultivation with selective media. Analysis of repetitive-element PCR （rep-PCR） genomic DNA fingerprinting identified 24 silicate bacteria from them. Determination of their potassium-releasing activities indicated that 7 isolates, i.e. FK2, FK3, FK8, FK11, FK4＇, FK23 and PK18, had high potassium-releasing activities, among which FK2 had the highest potassium-releasing activity. Its available potassium increment reached 41.79%. Observations on morphological features of bacterial colonies and phylogenetic analysis of 16S rDNA sequences to the 7 isolates with high potassium-releasing activity revealed that FK2 belonged to Pseudomonas sp., FK3 and FK23 belonged to Sinorhizobium sp., FK11 and FK8 belonged to Rhizobium sp., FK4＇ belonged to Mesorhizobium sp., and PK18 belonged to Flexibacter sp.

关 键 词：桑树 硅酸盐细菌 rep—PCR DNA指纹分析 解钾能力 分类 菌落形态 16S rDNA 

分 类 号：S888.2[农业科学—特种经济动物饲养]