cyclin B1的启动子在乏氧条件下活性的变化  

Regulation of promoter of cyclin B1 in cyclin B1 protein

作　　者：王昱[1] 卢仲毅[2] 朱宇熹[3] 

机构地区：[1]第三军医大学西南医院儿科,重庆400038 [2]重庆医科大学附属儿童医院PICU,400014 [3]重庆医科大学附属第一医院肿瘤科,400016

出　　处：《重庆医学》2010年第8期930-932,共3页Chongqing medicine

摘　　要：目的探讨细胞周期素B1(cyclin B1)的启动子在乏氧条件下活性的变化。方法采用PCR法获得对Hela细胞cy-clin B1的启动子,通过基因重组插入pGL3 promoter vector从而获得表达。荧光素酶的活性检测,了解通过双萤光素酶活性检测分析cyclin B1的启动子在乏氧条件下活性的变化。结果双萤光素酶活性检测cyclin B1的启动子活性在乏氧条件下,表达明显下降。结论在乏氧条件下,Hela细胞的cyclin B1的启动子活性下降,可能是乏氧条件下肿瘤细胞的自我保护机制。Objective To explore the activity of cyclin B1 promoter in hypoxic condition. Methods Constructed the plasmid of pGL3/cyclin B1 promoter. Transiented transfect the plasmid of pGL3/cyclin B1 promoter into HeI.a cells,and checked the activity of it. Then checked the activity of pGL3/cyclin B1 promoter in IteLa cell by dual lueiferase assay in hypoxia. Results The activity of cyclin B1 promoter was much lower in hypoxia than that in normoxia. Conclusion The activity of cyclin B1 promoter decreases in hypoxia, which may relate to the self protection of cell.

关 键 词：乏氧 细胞周期素B1 启动子 HELA细胞 萤光素酶 

分 类 号：R730.2[医药卫生—肿瘤]