余甘子提取物体外抗单纯疱疹病毒1型和2型的初步研究  

Study on the Anti-HSV Activity of Crude Extract from Phyllanthus emblica in vitro

作　　者：瞿畅[1,2,3] 赖志才[1,2,3] 裴赢[1,2,3] 张美英[2,3] 王一飞[2,3] 张颖君[4] 杨崇仁[4] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]暨南大学生物医药研究开发基地,广东广州510632 [3]基因工程药物国家工程研究中心,广东广州510632 [4]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《时珍国医国药》2010年第4期784-786,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.NSFCU0632010);植物化学与西部植物资源持续利用国家重点实验室自主课题(No.O807E21211);植物化学与西部植物资源持续利用国家重点实验室开放课题(No.O807B11211)

摘　　要：目的从余甘子的醋酸乙酯提取物、甲醇提取物和水提物3种提取物体外筛选抗单纯疱疹病毒1型和2型的活性成分。方法通过观察药物毒性、病毒引起的细胞病变效应、空斑减数法,判断药物的毒性及其抗病毒效应。结果余甘子的醋酸乙酯提取物、甲醇提取物和水提物对于单纯疱疹病毒1型的半数抑制浓度分别为25.28,66.17,100.94μg/ml;余甘子的3种提取物对于单纯疱疹病毒2型的半数抑制浓度分别为31.70,180.3,112.1μg/ml。结论余甘子3种提取物在体外对单纯疱疹病毒1型和2型均有一定的抑制作用。Objective To study the active components of anti - HSV - 1 and HSV - 2 of crude extract from Phyllanthus emblica. Methods The toxic and antivirous effects were evaluated by observing toxic effect, cytopathic effect（CPE） , MTT colorimetrie assay for vital cell rate, plaques reduction ratio. Results Inhibition on herpes simplex virus were observed during the experiment. The IC50 on HSV - 1 of acetic ether extract, methanol extract, aqueous extract from Phyllanthus emblica were 25.28, 66. 17, 100.94μg/ml ; while the IC50 on HSV - 2 of the extracts above were 31.70, 180.3, 112.1 μg/ml. Conclusion The three extracts from Phyllanthus emblica can inhibit both of HSV - 1 and HSV - 2 in vitro.

关 键 词：余甘子 单纯疱疹病毒1型 单纯疱疹病毒2型 细胞病变效应法 空斑减数法 

分 类 号：R284.2[医药卫生—中药学]