正交实验优选金花茶种子总多酚的最佳提取工艺  

Optimum Technology for Extraction of Total Polyphenol from Theopsis Chrysantha Hu.Seeds by Orthogonal Test

作　　者：唐前[1] 罗燕英 唐玲[1] 黄连冬 于大永[1] 冯宝民[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622 [2]广西南宁市金花茶公园,广西南宁530022

出　　处：《时珍国医国药》2010年第4期792-793,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30772714)

摘　　要：目的优选金花茶种子中总多酚的提取工艺。方法采用丙酮-水法从金花茶种子中提取总多酚,利用正交实验优选总多酚最佳提取工艺,考察丙酮浓度、时间、温度、提取次数及料液比对总多酚收率的影响。结果总多酚的最佳提取工艺为:25倍量的50%丙酮-水,80℃,提取3次,4 h/次,总多酚含量为9.59%,总多酚的收率为1.71%。结论丙酮-水提取法可用于金花茶种子中总多酚的提取,其中提取溶剂倍量和提取温度对总多酚提取的影响较大。Objective To optimize the technology for extraction of total polyphenol from Theopsis chrysantha Hu. Seeds. Methods The seeds was extracted using acetone - aqueous, and the extract rate of total polyphenol was studied by orthogonal test, and the effects of solvent concentration of extraction, time of extraction, temperature of extraction, number times of extraction, solvent times of extraction were investigated. Results The optimum extraction technology was as follows： 25 times 50% acetone -aqueous, extracting 3 times at 80℃ for 4h once. By this technology the extraction rate of total polyphenol was 1.71% and the content of total polyphenol was 9.59%. Conclusion This method can be applied the extraction of total polyphenol in Theopsis ehrysantha Hu. Seeds. The extracting solvent times and temperature of extraction show objective effects.

关 键 词：金花茶种子 总多酚 提取工艺 正交实验 

分 类 号：R284.2[医药卫生—中药学]