体外纯化乳鼠许旺细胞的4种方法比较(英文)  

Schwann cells purification by four different methods in vitro

作　　者：陈钢[1] 杨彩虹[2] 田林强[2] 郭风劲[2] 陈安民[2] 孙凯[2] 

机构地区：[1]江西省人民医院骨一科,江西省南昌市330006 [2]华中科技大学同济医学院附属同济医院骨科,湖北省武汉市430030

出　　处：《中国组织工程研究与临床康复》2010年第10期1892-1896,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江西省自然科学基金资助项目(2007GZY0589)~~

摘　　要：背景:许旺细胞是外周神经系统中修复神经损伤及神经疾病的主要种子细胞之一。但是,许旺细胞的来源有限,且由于成纤维细胞的污染,使许旺细胞的纯度受到影响。有学者提出了其他的纯化方法,但每种方法都存在其不足之处而不能很好的满足临床应用的需要。目的:比较差速贴壁消化法、冷喷注法、磁珠分选法和G418筛选法4种体外纯化乳鼠许旺细胞方法的差异。方法:取SD大鼠坐骨神经得到所需神经组织。分别用差速贴壁消化法、冷喷注法、磁珠分选法和G418筛选法纯化许旺细胞。比较4种方法获得细胞的活力,免疫组织化学法鉴定许旺细胞并计算纯度。结果与结论:差速贴壁消化法所得到的细胞纯度稍低,活力尚可;冷喷注法获得的细胞纯度和活力均较高;磁珠分选法获得的细胞纯度和冷喷注法相当,但是活力稍差;G418筛选法活力差,纯度高。BACKGROUND： Schwann cell is one of the major seed cells in peripheral nervous system and plays an important role in neural injury and neural disease. However, the source of Schwann cells is limited. And the purity of Schwann cells is affected due to the pollution of fibroblasts. Many purified methods have been proposed, but every one has its defect to satisfy the clinical demand. OBJECTIVE： To compare the differences among differential adhesion purified method, cold jet purified method, immunomagnetic beads selection purified method and G418 selection purified method to purify Schwann cells of neonatal rat in vitro. METHODS： Bilateral sciatic nerves of SD rats were harvested under sterile condition. Schwann cells were purified respectively using differential adhesion purified method, cold jet purified method, immunomagnetic beads selection purified method and G418 selection purified method. Cell viability was compared, and cell purity was determined by immunohistochemistry. RESULTS AND CONCLUSION： The purity of Schwann cells separated by differential adhesion method was low, but the viability was fair. The purity and viability of cells following cold jet method immunomagnetic beads selection method was high. The purity of cells separated by immunomagnetic beads selection methods was similar to that of cold jet method immunomagnetic beads selection method, but the cell viability was worse. The cell viability following G418 selection method was bad, but the purity was high.

关 键 词：许旺细胞 差速贴壁消化 冷喷注 磁珠分选 G418筛选 

分 类 号：R394.2[医药卫生—医学遗传学]