金属离子对红白锦鲤肠及肝胰脏蛋白酶活性的影响  

Effects of Metal Ions on Protease Activities in the Intestines and Hepatopancreas of Red-white Ornamental Carp

作　　者：郭永军[1] 魏东[1] 白东清[1] 闫珊珊[1] 吴旋[1] 宁博[1] 陆建权[1] 

机构地区：[1]天津农学院水产科学系天津市水产生态及养殖重点实验室,天津300384

出　　处：《安徽农业科学》2010年第10期5136-5137,5151,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究金属离子对红白锦鲤消化组织蛋白酶活性的影响。[方法]以酶学分析方法研究4种金属离子(K+、Na+、Mg2+、Ca2+)对红白锦鲤肝胰脏、前肠、中肠、后肠蛋白酶活性的影响。[结果]在设定的浓度范围内(25～150mmol/L),4种金属离子对蛋白酶活性影响不同。K+对肝胰脏和后肠的蛋白酶活性有不同程度的促进作用,其中K+对中肠表现为低浓度促进,高浓度抑制的作用,对前肠蛋白酶表现为抑制作用;Na+对肝胰脏、前肠和后肠的蛋白酶表现为不同程度促进作用,对中肠表现为抑制作用。Mg2+、Ca2+对肠及肝胰脏的蛋白酶均表现为不同程度的抑制作用。[结论]该研究可为红白锦鲤消化生理的研究以及配合饲料的开发、优化提供基础数据和理论依据。[Objective]The research aimed to study the effects of metal ions on the protease activities in digestive tissue of red-white ornamental carp.[Method] The effects of four kinds of metal ions （K＋,Na＋,Mg2＋,Ca2＋） on protease activities in hepatopancreas,foregut,midgut,hindgut of red-white ornamental carp were studied by enzyme analysis method.[Result]The effects of four metal ions on protease activities of red-white ornamental carp were different in the range of experimental concentration form 25 mmol/L to 150 mmol/L.K＋could promote protease activities in hepatopancreas and hindgut at different degree,K＋ had promoting effect at low-concentration,but inhibitory effect at high-concentration in midgut,inhibitory effect in foregut.Na＋ had promoting effect on protease in hepatopancreas,foregut and hindgut at different degree,but inhibitory effect in midgut.Mg2＋ and Ca2＋ had inhibitory effect on protease in intestinal and hepatopancreas at different degree.[Conclusion]The research provided theoretical basis for the research on the digestive physiology of red-white ornamental carp and the development and optimization of compound feed.

关 键 词：红白锦鲤(Cyprinus carpio L.) 金属离子 蛋白酶活性 

分 类 号：S917.4[农业科学—水产科学]