结外B细胞性非霍奇金淋巴瘤中MALT1基因表达的检测及意义  

Clinical Significance of MALT1 Gene Detection in Extra-nodal B Cell Lymphoma

作　　者：郭宏伟[1] 杨文秀[2] 钟愉[2] 陈玉梅[2] 王松[2] 刘启兰[2] 刘辉[2] 

机构地区：[1]山东省临沂市人民医院病理科 [2]贵阳医学院病理科,贵阳市550004

出　　处：《中国肿瘤临床》2010年第6期320-322,326,共4页Chinese Journal of Clinical Oncology

基　　金：贵州省卫生厅科技计划项目(编号:G2005-2);贵州省省长基金资助项目(编号:s2004-18);贵阳医学院附属医院博士启动基金资助(编号:C2005-1)~~

摘　　要：目的:探讨结外B细胞淋巴瘤中MALT1基因(mRNA)的表达与MALT和弥漫大B细胞淋巴瘤发生发展的关系,及其对淋巴瘤临床病理特征和预后的影响。方法:用HE和免疫组化染色筛选出本院确诊的MALT和结外弥漫大B细胞淋巴瘤106例,检查肿瘤组织Bcl-2和Ki-67的表达,用RT-PCR检测肿瘤中的MALT1基因mRNA水平,收集临床病理资料并随访。结果:MALT1 mRNA的表达:在DLBCL与MALT淋巴瘤组、淋巴结累及组和无累及组、临床分期Ⅲ～Ⅳ组和Ⅰ～Ⅱ组间均有统计学差异(P<0.05),在Ki-67≥30%与<30%组间未见统计学差异(P>0.05),而Bcl-2阳性组却高于阴性组(P<0.05);MALT1mRNA表达与不表达病例之间患者生存状况有明显差异(P<0.05),表达组生存状况较差。结论:MALT1基因在MALT淋巴瘤与DLBCL中有不同程度的表达,MALT1基因活化NF-κB后可能通过调控Bcl-2使细胞凋亡抑制而导致淋巴瘤发生,MALT1mRNA表达与B细胞淋巴瘤的类型、淋巴结累及、临床分期有关,与患者的生存状况有关,MALT1可能是一个MALT和弥漫大B细胞淋巴瘤预后不良的相关因子。Objective： To investigate the relationship between MALT1 mRNA expression in extranodal B cell lymphoma and the pathogenesis of MALToma and DLBCL, and to explore the effect of MALT1 mRNA expression on the clinicopatho-serve the expression of Bcl-2 and Ki-67 in 106 samples of extranodal B cell lymphoma.The mRNA of MALT1 was detected by RT-PCR.Clinicopathological data were reviewed.Follow-up and statistical analysis were performed.Results： Expression of MALT1 mRNA was statistically different between the cases with lymph node metastasis and those without lymph node mestastasis, and between staging Ⅰ-Ⅱ cases and stage Ⅲ-Ⅳ cases, The expression of MALT1 mRNA in cases with posi-tive expression of MALToma, DLBCL, and Bcl-2 was higher than in those without expression of the three indices （P〈0.05）.However, no significant difference was found in MALT1 mRNA expression between cases with Ki-67≥30% and those with Ki-67〈30% （P〉0.05）.MALT1 mRNA-positive cases showed a worse survival status than MALT1 mRNA-negative cases （P〈0.05）.Conclusion： Expression of MALT1 gene is different between MALToma and DLBCL.Inhibition of apoptosis by Bcl-2 overexpression caused by activation of NF-κB may lead to the pathogenesis of MALToma and DLBCL.The expression of MALT1 mRNA is associated with the types of lymphoma, involvement of lymph node, stage of lymphoma, and patient sur-vival.The expression of MALT1 gene may be associated with poor prognosis of MALToma and DLBCL.

关 键 词：MALT1基因 淋巴瘤 RT-PCR 预后 

分 类 号：R733.1[医药卫生—肿瘤]