四种牙周致病菌侵入血管内皮细胞能力的体外研究  

Invasion of four common periodontal pathogens into vascular endothelial cells in vitro

作　　者：邓辉[1] 吴亚菲[1] 丁一[1] 苗棣[1] 高雳[1] 郭淑娟[1] 

机构地区：[1]四川大学华西口腔医学院牙周病科,成都610041

出　　处：《中华口腔医学杂志》2010年第4期203-206,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30973323）;“十一五”国家科技支撑计划（2007BA118802）;浙江省高校科研计划（Y200803098）

摘　　要：目的研究和比较4种常见牙周致病菌Pg33277、Pi25611、Aa29522和Fn10953黏附和侵入人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVEC）的能力，为深入探讨牙周致病菌感染HUVEC在促进动脉粥样硬化发生、发展中的作用奠定初步基础。方法建立牙周致病菌感染HUVEC的体外模型，采用扫描电镜、抗生素保护一菌落计数法观察以上牙周致病菌黏附和侵入HUVEC的能力。结果扫描电镜结果表明P酚3277、Pi25611、Aa29522和Fnl0953均可黏附于HUVEC；抗生素保护法结果发现Pg33277、Pi25611、Aa29522和Fn10953均可侵入HUVEC，细菌侵入量分别为（0．8±0．1）×10^8、（4．1±0．5）×10^6、（1．6±0．3）×10^6、（5．0±0．4）×10^6CFU／L，侵入率分别为（0．400±0．050）％、（0．021±0．003）％、（0．008±0．002）％和（0．025±0．002）％，Pg33277侵入能力远较其他3种牙周致病菌强（P〈0．001），其余3种牙周致病菌问的侵入能力差异无统计学意义（P〉0．05）。结论4种常见牙周致病菌Pg33277、Pi5611、Aa29522和Fn10953均可黏附和侵入HUVEC，pg33277侵入能力最强，这可能为其进一步发挥相关的生物学作用奠定基础。Objective To investigate the adhesive and invasive ability of four common periodontal pathogens, Pg33277, Pi25611, Aa29522 and Fn10953 in human umbilical vein endothelial cells（HUVEC）. Methods The model of infection of HUVEC by periodontal pathogens was established in vitro. The invasive ability of four periodontal pathogens in HUVEC was tested by scanning electron microscope （SEM） and antibiotic protection assays-colony-forming units （CFU）. Results All of the four periodontal pathogens were found to adhere to HUVEC by SEM and invaded HUVEC at invasion numbers of （0. 8 ± 0. 1 ） ×10^6, （4. 1 ± 0. 5） ×10^6, （ 1.6 ± 0. 3 ） ×10^6 and （ 5. 0 ± 0. 4 ） ×10^6CFU/L respectively by antibiotic protection assays-CFU. The invasion efficiencies were （0. 400 ±0. 050）%, （0. 021 ±0. 003）%, （0. 008 ±0. 002）% and （0. 025 ±0. 002）%, respectively. The invasive ability of Pg33277 was significantly greater than those of the other three periodontal pathogens （P 〈 0. 001 ）. There was no difference in invasive abilities among Pi25611, Aa29522 and Fn10953 （P 〉 0. 05）. Conclusions All of the four common periodontal pathogens, Pg33277,Pi25611 ,Aa29522 and Fn10953 could adhere to and invaded HUVEC, with Pg33277 being the strongest.

关 键 词：牙周炎 内皮细胞 细菌黏附 侵入 

分 类 号：R781.4[医药卫生—口腔医学]