测定豆类胰蛋白酶抑制剂活性和种类的电泳方法改良  

An Improved Electrophoresis Method for Determining Trypsin Inhibitors Activities and Variety from Bean

作　　者：黄国伟[1] 巫光宏[1] 何嘉雯[1] 吴奕文[1] 陈晓峰[1] 

机构地区：[1]华南农业大学生命科学学院,广东广州510642

出　　处：《大豆科学》2010年第2期315-318,共4页Soybean Science

基　　金：华南农业大学大学生科技创新资助项目(L06056)

摘　　要：以赤豆胰蛋白酶抑制剂(ABTI)及绿豆的胰蛋白酶抑制剂(MBTI)为材料,采用明胶-PAGE技术对其进行活性和种类检测。同时对影响明胶-PAGE技术体系的明胶浓度、酶解时间、脱酶液及电泳的灵敏度等因素进行探讨。结果表明:改良后的明胶-PAGE技术,鉴定胰蛋白酶抑制剂活性和种类的时间大大缩短,成本低,技术操作可行性强,重复性好,且检测ABTI的种类达7种,灵敏度达到每孔1.0 BAEE、蛋白点样量为每孔0.154μg。Trypsin inhibitor （TI） , a major anti-nutritional factor, it＇s variety and activities provided an important reference for the eradication of anti-nutritional factors in diet bean. In this study, we determined the activities and variety of Adzuki Bean Trypsin Inhibitor（ABTI） and Mung Bean Trypsin Inhibitor（MBTI） by means of gelatin-PAGE techniques; and explored the various factors of affecting the gelatin-PAGE, including gelatin concentration, enzyme hydrolysis time, the sector of rinse enzyme solution and the sensitivity of electrophoresis. The results showed that the improved gelatin-PAGE could detect 7 types of ABTI, its sensitivity reached 1.0 BAEE·hole^-1 with the protein sampling volume of 0. 154μg·hole^-1. The improved method had many advantages such as time and cost saving, easy to operate and reproducible.

关 键 词：胰蛋白酶抑制剂 明胶-PAGE 灵敏度 

分 类 号：R151[医药卫生—营养与食品卫生学]