呼吸商监控毕赤酵母表达猪α干扰素活性水平的研究  

Porcine interferon-α expression with Pichia pastoris fed-batch cultivation under respiratory quotient monitoring

作　　者：徐俊[1] 金虎[1] 高敏杰[1] 虞瑞嵩[2] 董世娟[2] 段作营[1] 史仲平[1] 李震[2] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122 [2]上海市农业科学院畜牧兽医研究所,上海201000

出　　处：《生物学杂志》2010年第2期5-8,共4页Journal of Biology

基　　金：国家高技术研究发展计划863计划(No.2006AA020101);国家自然科学基金(No.60604028);国家重点基础研究发展计划973计划(No.2007CB714306)资助;上海市科学技术委员会农业科技重点项目(No.073919108)

摘　　要：在5L发酵罐下开展毕赤酵母流加培养表达pIFN-α研究。通过多批次研究,结果表明:诱导阶段,RQ稳定情况可以作为反映底物甲醇浓度水平是否合适以及pIFN-α活性高低的监控指标。当甲醇浓度在8～12g/L水平时,RQ在整个诱导阶段能稳定在0.4～0.5之间某个特定值上,同时pIFN-α最高活性都能维持在106IU/mL水平上;而当甲醇浓度在0～5g/L或15～20g/L范围内时,RQ很不稳定,分别在0.15～0.4和0.4～0.8区间上下振动,相应的pIFN-α最高活性也只在103IU/mL和104IU/mL水平上。Porcine interferon-α （pIFN-α） expression under high density recombinant Pichia pastoriscultivation was conducted in a 5L bench-scaled bioreactor.The results indicated that the stability of respiratory quotient （RQ） could reflect the suitability of methanol concentration for Pichia pastorisinduction expression and the pIFN-α activity level indirectly.Maintained with a moderate induction methanol concentration of 8～12g/L,RQ could keep constant at a certain value between 0.4 and 0.5 in the whole induction stage.Meanwhile,the highest pIFN-α activity could keep above 106IU/mL.When the methanol concentration was maintained at a lower level of 0～5g/L or a higher level of 15～20g/L,the RQ was unstable and the highest pIFN-α activities were at the level of 10^3IU/mL and 10^4IU/mL,respectively.

关 键 词：毕赤酵母 猪Α干扰素 呼吸商 流加培养 

分 类 号：Q819[生物学—生物工程]