X射线辐照后人肝细胞、肝癌细胞存活与端粒酶活性关系的研究  

The relationship between cell survival rate and telomerase activity of the human hepatocellular cells and carcinoma cells irradiated by X-rays

作　　者：刘金婷[1,2] 党秉荣[1] 李文建[1] 荆西刚[1] 魏巍[1] 王转子[1] 丁楠[1] 高清祥[3] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院研究生院,北京100049 [3]兰州大学生命科学院,兰州730000

出　　处：《辐射研究与辐射工艺学报》2010年第2期119-123,共5页Journal of Radiation Research and Radiation Processing

摘　　要：利用X射线对体外培养的人肝癌细胞SMMC-7721和正常人肝细胞HL-7702进行辐照,以细胞克隆形成法测定细胞存活率,多聚酶链式反应银染端粒重复序列扩增法(Telomeric repeat amplification protocol,TRAP-PCR)检测细胞中端粒酶活性变化,探讨辐照后细胞存活与细胞中端粒酶活性变化的关系。结果表明,SMMC-7721和HL-7702细胞的存活率都随辐照剂量的增加而相应降低。在1-4Gy剂量范围内,两种细胞的端粒酶活性变化均呈剂量依赖性增强。但在1Gy、2Gy、3Gy剂量下,SMMC-7721其端粒酶活性较对照(0Gy)细胞低;当辐照剂量达4Gy时,端粒酶活性略高于对照细胞。在1-4Gy剂量范围内,HL-7702和SMMC-7721的细胞存活与端粒酶活性在变化趋势上呈负的关系。In this paper,the relationship between cell survival fraction and telomerase activity was investigated with the human hepatocellular cells HL-7702 and carcinoma cells SMMC-7721 irradiated by X-rays. The colony formation assay and TRAP （Telomeric repeat amplification protocol） method were used to determine the cell survival fraction and telomerase activity respectively. To both of HL-7702 and SMMC-7721,the telomerase activity was increased in dose-dependent. However,at 1—3 Gy,the telomerase activity was lower than that of control in SMMC-7721,and it was a little higher than that of control when irradiated at 4 Gy. At 1—4 Gy,the variation trend of cell survive and that of telomerase activity were identical.

关 键 词：X射线 细胞存活率 端粒酶活性 SMMC-7721 HL-7702 

分 类 号：Q691[生物学—生物物理学] R730[医药卫生—肿瘤]