XPD基因多态性与原发性肝癌危险性的病例对照研究  

A case-control study on the polymorphism of gene XPD and the susceptibility of primary hepatic carcinoma

作　　者：崔铉默[1,2] 苏红英[2] 

机构地区：[1]鞍山市中心医院介入科,辽宁鞍山114000 [2]中国医科大学附属第一医院介入科

出　　处：《中国民康医学》2010年第8期912-914,994,共4页Medical Journal of Chinese People’s Health

摘　　要：目的:探讨DNA修复基因XPD多态性与原发性肝癌易感性的关系。方法:对实验组(辽宁地区94例原发性肝癌)及正常对照组(111例)采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法,检测XPD基因Lys75lGln多态基因型。结果:94例肝癌患者XPD751基因Lys/Lys,Lys/Gln,Gln/Gln多态基因型频率分别为69例(73.4%),24例(25.5%)和1例(1.1%),而111例健康对照组分别为97例(87.4%)、14例(12.6%)和0例(0%);两组间比较有非常显著差异。经Logistic回归分析发现,携带至少1个XPD751Gln等位基因(Lys/Gln和Gln/Gln基因型)的个体患肝癌的危险显著增高。结论:XPD751位点基因多态性与原发性肝癌的发生有一定相关,XPD基因Lys751Gln多态性是原发性肝癌的遗传易感因素。Objective： To evaluate the correlation between the polymorphism of DNA repair gene XPD and risk of primary hepatic carcinoma （PHC）. Methods： The experimental subjects composed of 94 cases with PHC and 111 healthy controls in Lianning Province. The polymorphism genetic type of Lys751Gln of gene XPD were analyzed by PCR- BFLP.Rawlls： Tbe frequence of polymorphism genotype of Lys/Lys, Lys/Gln, Gin/Gin of XPD751 gene in patients with PHC were 69（73.4%）,24（25.5%） and 1（1.1%）respectively, which in the control group were 97（87.4%）, 14 （ 12.6% ） and 0（0% ）. The difference were highly significant between the groups. It was observed by Logistic regression analysis that the risk of suffer- ing PHC increased significantly in individuals carrying at least one allele of 751Gin（ Lys/Gln 和 Gin/Gin genotype）. Conclusion： Our study discovered that XPD 751 site gene polymophism is related to PHC closely. The pelymophism of XPD gene Lys751Gin is a factor of hereditary susceptibility in PHC.

关 键 词：原发性肝癌 DNA修复基因 XPD 基因多态性 遗传易感性 

分 类 号：R735.7[医药卫生—肿瘤]