谷氨酰胺联合5-氟尿嘧啶和环磷酰胺对胃癌细胞增殖的影响  

Effect of combination of Glutamine (Gln) and 5-Fluorouracil (5-FU) and Cyclophosphamide (CTX) on the proliferation of human gastric carcinoma cells

作　　者：韩建立[1] 赵浩亮[1] 杨海波[1] 韩振国[1] 王贵明[1] 刘宇[1] 

机构地区：[1]山西医科大学第一医院,山西太原030001

出　　处：《中国医药导报》2010年第12期26-28,共3页China Medical Herald

摘　　要：目的:研究谷氨酰胺(Gln)联合5-氟尿嘧啶(5-FU)和环磷酰胺(CTX)对人胃癌SGC7901细胞周期和凋亡率的影响。方法:体外培养胃癌SGC7901细胞株,加入Gln及不同浓度的5-FU和CTX后收集细胞。应用流式细胞术检测胃癌细胞周期及凋亡率的变化。结果:5-FU加Gln时胃癌SGC7901细胞凋亡率不同,浓度由0mg/L增加到240mg/L时呈正直线相关(r=0.806,P<0.01),凋亡率随浓度增加而增加。CTX加Gln对胃癌细胞周期及凋亡率无显著影响(P>0.05)。5-FU加Gln组G0/G1期细胞所占比例较CTX加Gln组明显增多(P<0.05)。G2期5-FU加Gln组明显比CTX加Gln组低(P<0.05)。S期两组比较,差异无统计学意义(P>0.05)。结论:Gln和5-FU影响胃癌细胞株的细胞周期和凋亡率。Gln和CTX对人胃癌细胞株的细胞周期和凋亡率均无明显影响。Objective：To evaluate the effect of combination of Gln and 5-FU and CTX on the human gastric carcinoma cell line SGC7901 in vitro. Methods：Different concentrations of 5-FU or CTX and Gln were added in the cultured human gastric carcinoma cell line SGC7901 in vitro. The changes of apoptosis rate and cell cycle were detected by the flow cy-tometry analysis. Results：Combination of Gln and 5-FU could inhibit the cell growth and enhanced apoptosis rate. The concentration of 5-FU and the apoptosis rate of gastric carcinoma cell line SGC7901 had positive linear correlation （r=0.806,P0.01）,the apoptosis rate increased with the increase of concentration. There was no change of apoptosis rate and cell cycle by combination of CTX and Gln （P0.05）. The rate of G0/G1 phase in cell cycle of 5-FU and Gln group was more than that of CTX and Gln group （P0.05）. The rate of G2 phase in cell cycle of 5-FU and Gln group was less than that of CTX and Gln group （P0.05）. The rate of S-phase in cell cycle had no difference in two groups （P0.05）. Conclusion：Combination of Gln and 5-FU can inhibit the cell growth and enhance apoptosis rate. There is no change of apoptosis rate and cell cycle by combination of CTX and Gln.

关 键 词：谷氨酰胺 细胞周期 凋亡 细胞培养 

分 类 号：R73[医药卫生—肿瘤]