用rpsL基因突变实验评价丙烯酰胺致突变性的方法初探  

Application of rpsL Mutation Assay for Mutagenicity with Acrylamide

作　　者：刘清岱[1] 王志伟[1] 安红杰[1] 张治洲[1] 

机构地区：[1]天津科技大学食品科学与生物技术学院,天津300457

出　　处：《食品与生物技术学报》2010年第2期298-301,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30800255);教育部新世纪优秀人才计划;天津科技大学人才引进基金项目(20060432;20060424)

摘　　要：采用rpsL基因突变实验对丙烯酰胺的致突变性进行了初步研究。实验设0.05、0.10、0.25、0.50、1.00 mg/mL 5个丙烯酰胺的剂量组和阴性、阳性对照组。结果发现,丙烯酰胺剂量组的突变频率与阴性对照组相比均有显著差异,并且有剂量效应关系,表明丙烯酰胺可诱发rpsL位点的突变。由于rpsL突变实验自发突变背景水平低、灵敏度高,因此rpsL突变实验有望成为食品和医药领域中致癌物快速检测的有效工具。In this study,rpsL mutation assay was use as model to determine the mutagenicity induced by acrylamide.E.coli MF101 carried with pMOL21 was treated with different concentrations of acrylamide（0.05,0.10,0.25,0.50,1.00 mg/mL,respectively）.The results showed that with the increasing of the acrylamide concentration,rpsL mutation frequency significantly increases.Furthermore,a dosage-dependent relationship was detected.The results present here strong suggested that the rpsL mutation assay may be as a potential tool for investigating food and drug safety.

关 键 词：rpsL基因突变 丙烯酰胺 致突变性 

分 类 号：R155[医药卫生—营养与食品卫生学]