三氧化二砷联合全反式维甲酸对人卵巢癌细胞的抑制作用  

Inhibition of arsenic trioxide combined with atra to human ovarian cancer cell

作　　者：赵静[1] 封全灵[1] 乔丽娟[1] 

机构地区：[1]郑州大学第三附属医院,河南郑州450052

出　　处：《中国妇幼保健》2010年第11期1545-1548,共4页Maternal and Child Health Care of China

基　　金：河南省医学科技攻关计划基金资助项目〔200703070〕

摘　　要：目的:探讨三氧化二砷(AS2O3)联合全反式维甲酸(ATRA)对人浆液性卵巢癌细胞株SKOV3的抑制作用及其机制。方法;四甲基偶氮唑蓝光吸收法(MTT)、流式细胞术及免疫荧光法检测体外培养的SKOV3细胞在AS2O3单独作用及联合ATRA作用下对卵巢癌细胞生长的抑制及促凋亡作用。结果:单用ATRA对SKOV3细胞的生长无影响;单用AS2O3可以抑制SKOV3细胞的生长;联合使用AS2O3和ATRA较单用AS2O3对SKOV3细胞的抑制作用强(P<0.05);流式细胞术证实:5.0μmol/LAS2O3联合1.0μmol/LATRA作用48h时,SKOV3细胞出现G2/M期阻滞增强、S期减低(P<0.05);AnnffxinV染色检测到早期凋亡细胞增多,晚期凋亡及继发性坏死细胞减低(P<0.05)。结论:AS2O3能上调SKOV3细胞中细胞周期抑制蛋白P27的表达,ATRA联合AS2O3可以增强上述效应。故ATRA对诱导SKOV3细胞凋亡有增敏效应,该效应过程可能依赖于细胞周期负性调控因子P27蛋白的过度表达。Objective： To investigate the effects of arsenic trioxide and all - trans retinoic acid （ATRA） on ovarian cancer cell line SKOV3 and relative mechanism. Methods ： Morphological changesof cells were observed and photoed by inverted microscope. Growth inhibition and eell cycles change of SKOV3 line induced by means of MTT/flow cytometry. Immunocytochemical staining was used to examine the expression of P27. Results： The inhibitive effect of arsenic trioxide （5.0 μmol./L） combined with ATRA （1.0 μmol/L） was obvious and the inhibitive degree was stronger than only arsenic trioxide. Higher concentrations of arsenic trioxide and all - trans retinoic acid up - regulated P27 . Conclusion ： The inhibitive effect of arsenic trioxide combined with ATRA is more obvious than either one used separately ; G2/M retardation is found by flow cytometry and the effect is enhanced in dose - dependent pattern. The apoptosis mechanism of ovarian cancer cell line SKOV3 is related with up - regulating of P27.

关 键 词：三氧化二砷 全反式维甲酸 人浆液性卵巢癌SKOV3细胞株 细胞周期抑制蛋白(P27蛋白) 

分 类 号：R737.31[医药卫生—肿瘤]