鹿茸的HPLC指纹图谱  

Fingerprint of Pilose Antler by HPLC

作　　者：阎正[1] 于趁[1] 张智慧[1] 蔡立鹏[1] 王春云[1] 苑若瑶[1] 王继坤 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]北京中防吴通防伪科技中心,北京100025

出　　处：《河北大学学报（自然科学版）》2010年第2期164-170,共7页Journal of Hebei University(Natural Science Edition)

基　　金：国家科技支撑项目（2006BAK02A17）

摘　　要：建立了鹿茸的高效液相指纹图谱.研究了不同的预处理方法、流动相组成、最佳检测波长等操作因素对鹿茸指纹谱图的影响,并对6种不同产地的鹿茸药材的指纹图谱进行了比较.色谱条件为：InertsilODS-3色谱柱（250 mm×4.6 mm;5μm）;检测波长270 nm;流动相为甲醇-磷酸水溶液（质量分数为0.05%）梯度洗脱;流速：1 mL/min;分析时间：100 min;柱温为25℃.This paper described an analytical method of fingerprint of pilose antler by HPLC.different sample pretreatment,detection wavelength,mobile phase,temperature were studied and the fingerprint chromatograms of pilose antler from six different areas were compared.Inertsil ODS-3 column（250 mm×4.6 mm,5 μm,） was used with the mixture of methanol-phosphoric 0.05% acid;the detection wavelength was 270 nm;the flow rate was 1 mL/min and the column temperature was 25 ℃.

关 键 词：鹿茸 HPLC 指纹图谱 

分 类 号：O657.7[理学—分析化学]