新月弯孢霉KA-91转化异羟基洋地黄毒苷元的研究  

Biotransformation of Digoxigenin by Curvularia Lunata KA-91

作　　者：刘增兵[1] 孙华[1] 和朝军[1] 付玉婉[1] 王敏[1] 

机构地区：[1]天津科技大学生物工程学院工业微生物教育部重点实验室,天津300457

出　　处：《化学与生物工程》2010年第4期57-60,共4页Chemistry & Bioengineering

基　　金：天津市应用基础及前沿技术研究计划重点项目(08JCZDJC15200)

摘　　要：考察了新月弯孢霉(Curvularialunata)KA-91对地高辛和异羟基洋地黄毒苷元的生物转化反应。结果发现,新月弯孢霉KA-91对地高辛不具有转化能力,对异羟基洋地黄毒苷元转化产生一个产物,经结构鉴定产物为3-羰基-异羟基洋地黄毒苷元。确定了转化异羟基洋地黄毒苷元反应的最优工艺参数为:发酵培养基的初始pH值6.0,装液量50 mL.(250 mL)-1,底物浓度0.60 mmol.L-1,转化温度28℃,180 r.min-1振荡培养24 h,转化54 h。此时,转化率为40%。Microbial transformations of digoxin and digoxigenin by Curvularia lunata KA-91 were investigated. Curvularia lunata KA-91 had the conversion capability of digoxigenin, except digoxin. The product was identified as digoxigenone by structure characterization. The optimum biotransformation conditions of digoxigenin were obtained as follows： initial pH value 6.0, the strain incubated in 250 mL shake flask with 50 mL medium at 28℃, substrate concentration 0. 60 mmol · L^-1 , culture for 24 h and biotransformation for 54 h at 180 r· min^-1. The biotransformation rate reached 40% under above conditions.

关 键 词：异羟基洋地黄毒苷元 新月弯孢霉 生物转化 条件优化 

分 类 号：TQ920[轻工技术与工程—发酵工程] R284[医药卫生—中药学]