黄瓜再生体系的建立  

The Regeneration System of Cucumber

作　　者：薛丹丹[1] 张凤生[1] 王保菊[1] 李建红[1] 赵娜[1] 武斌[1] 

机构地区：[1]哈尔滨市农业科学院,黑龙江哈尔滨150070

出　　处：《北方园艺》2010年第7期119-121,共3页Northern Horticulture

摘　　要：以津研4号为试材,利用组织培养技术探讨黄瓜子叶离体培养技术,建立高频的黄瓜再生体系;以MS为基本培养基,附加不同浓度和不同种类的激素,探讨影响黄瓜离体组织培养的诸多因素。结果表明:以黄瓜5～6 d苗龄无菌苗子叶作外植体,愈伤组织生长旺盛,芽诱导率高;7～8 d的外植体则生长势弱,不利再生芽的分化。6-BA4.0 mg/L+IAA0.3 mg/L,芽分化频率较高,芽的分化率可达83%;待再生芽长至2～3 cm时移入生根培养基(1/2MS+0.3 mg/LNAA),生根率可达88%,30～50 d可得到完整再生植株。In this study, Heilongjiang Cucumber Varieties Yan jing Four Number for test material, investigate cucumber cotyledons in vitro culture technique with tissue culture, and construct cucumber high-frequency regeneration system. The basic medium was MS, affixated different concentrations and different types of PGRs, and to explore the impact of cucumber many factors in vitro tissue culture. The results showed that 5~6 days in sterile cucumber cotyledons as explants, callus growth exuberant and the rate of bud inducted was high; 7~8 days in sterile cucumber cotyledons as explants, growth potential was weak and go against the differentiation of adventitious buds. When MS affix 0. 4 mg/L 6- BA and 0. 3 mg/L IAA, the frequency of adventitious bud differentiation was high and bud differentiation rate up to 83X;To be adventitious buds grows to 2~3 cm into the rooting medium, which was 1/2MS affix 0. 3 mg/L NAA. Rooting rate was 88% and 30~50 days can be a complete plant.

关 键 词：黄瓜 再生体系 子叶 不定芽 

分 类 号：S242.204.3[农业科学—农业电气化与自动化]