三种木兰科植物茎和叶提取物的抑菌活性研究  

Antimicrobial activity of stem and leaf extracts from three species of the Magnoliaceae

作　　者：李晓娜[1,2] 朱文清[1] 陈展慧[1] 冯晓光[1] 梅亚宁[3] 田兴军[1] 

机构地区：[1]南京大学生命科学学院,南京210093 [2]贵州师范大学中国南方喀斯特研究院,贵阳550001 [3]江苏省人民医院,南京210029

出　　处：《南京大学学报（自然科学版）》2010年第2期197-202,共6页Journal of Nanjing University（Natural Science）

基　　金：国家自然科学基金(30870419);贵州省科学技术基金(黔科合J字[2009]2284号);国家重点基础研究发展计划(973计划)(2008CB418000)

摘　　要：木兰科植物木莲(Manglietia fordiana)、含笑(Michelia figo)和北美鹅掌楸(Liriodendrontulipifera)是中国的传统药用植物.本文通过对抑菌圈大小和最小抑菌浓度的测定,研究了这3种植物茎、叶的乙醇粗提物的抑菌活性.结果表明,大部分提取物对革兰氏阳性菌有较好的抑制效果,个别提取物(如木莲茎和含笑茎)能抑制革兰氏阴性菌的生长,所有提取物均无抑制病原真菌的作用.Manglietia Jordiana, Michelia figo and Liriodendron tulipifera （Magnoliaceae） are traditional medicines in China. The antibacterial and antifungal activities of crude ethanolic extracts from stems and leaves of the three species were investigated using modified agar diffusion method and minimum inhibitory concentration （MIC） method. The results showed that all stem extracts of the three species were more active than leaf extracts. Most of the leaf extraets except Manglietia fordiana were proved to be active against Gram positive pathogenic bacteria Staphylococcus aureus, Nocardia asteroides and Bacillus subtilis. Gram positive pathogenic bacterium Streptococcus Jaecalis was only inhibited by the extract of Liriodendron tulipifera stem. Only a few extracts （stems of Manglietia fordiana and Michelia figo ） were active against Gram negative bacteria, All the extracts in this study could not inhibit Enterobacter cloacae. Stem extract of Manglietia fordiana was active to B. dysenteriae, Escherichia coli and B. pyocyaneus; Stem and leaf extracts of Michelia figo possessed relatively high antibacterial activity against Escherichia coli.

关 键 词：木兰科 提取物 病原菌 抑菌活性 

分 类 号：Q948.122.3[生物学—植物学]