大鼠肝纤维化组织内ROCK-I、磷酸化MBS Thr-697和α-SMA表达增强  

Increased expression of ROCK-I,p-MBS Thr-697 and α-SMA during hepatic fibrogenesis

作　　者：扈彩霞[1,2] 郑加生[1] 王玉珍[2] 姜慧卿[2] 

机构地区：[1]首都医科大学附属北京佑安医院肿瘤肝胆微创介入中心,北京100069 [2]河北医科大学第二附属医院消化内科,河北石家庄050000

出　　处：《基础医学与临床》2010年第4期355-359,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C2007000843)

摘　　要：目的探讨ROCK-I、磷酸化MBS Thr-697、α-SMA蛋白及其mRNA在实验性大鼠肝纤维化形成过程中的动态表达及肌动蛋白细胞骨架的变化。方法结扎胆总管制备肝纤维化模型,用免疫组织化学、Western blot与RT-PCR检测ROCK-I、α-SMA蛋白及其mRNA的表达,用特异性荧光染色检测肌动蛋白细胞骨架。结果随着肝纤维化的发展,大鼠肝脏中ROCK-I及α-SMA阳性细胞明显增多;造模1～4周,肝组织ROCK-I、α-SMA的阳性面积及ROCK-I、磷酸化MBS Thr-697、α-SMA蛋白表达显著高于正常组(P<0.05);ROCK-I基因表达与α-SMA表达呈正相关(r=0.718,P<0.05);随着造模时间延长,肝组织肌动蛋白荧光信号增强。结论ROCK-I在肝纤维化发生过程中表达上调和功能活化,可能通过诱导肌成纤维细胞表型形成而参与肝纤维化的发生。Objective To explore the dynamic expression of ROCK-I,p-MBS Thr-697,α-SMA protein and their mRNA in the hepatic fibrogenesis and the changes of actin cytoskeleton.Methods ROCK-I and p-MBS Thr-697 protein in liver were determined by Western blot and their mRNA was examined by reverse transcription-polymerase chain reaction（RT-PCR）,while the distribution of ROCK-I and α-SMA in liver was assessed immunohistochemistically.The change of actin cytoskeleton was shown by fluorescence.Results With the development of hepatic fibrosis,the positive areas in model groups at week 1 to 4 of ROCK-I and α-SMA of the rat livers were larger than that in control group respectively（P0.05）.ROCK-I,p-MBS Thr-697,α-SMA protein and mRNA were increased than that in control group respectively.ROCK-I mRNA expression correlated with α-SMA（r=0.718,P0.05）.With the development of liver fibrosis,the images of fluorescence were inhanced.Conclusion With the development of liver fibrosis,both protein and mRNA of ROCK-I increased.

关 键 词：肝纤维化 Rho相关卷曲螺旋形成蛋白激酶 细胞骨架 

分 类 号：R575.2[医药卫生—消化系统]