锂-匹鲁卡品诱导大鼠癫痫发作后海马齿状回IL-1 mRNA表达的变化  

Expression of IL-1 mRNA in the dentate gyrus of adult rats following lithium-pilocarione-induced seizures

作　　者：张世俊[1] 李晓伟[1] 王莹[1] 魏东[1] 江文[1] 

机构地区：[1]第四军医大学西京医院神经内科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2010年第3期288-290,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30870840);神经科学国家重点实验室开放性课题(2008)

摘　　要：目的:观察大鼠癫痫发作后海马齿状回细胞因子IL-1(IL-1R、IL-1β、IL-1ra)mRNA的表达变化,探讨IL-1在癫痫发作中的作用。方法:大鼠随机分为对照组和实验组。实验组大鼠腹腔注射氯化锂以及匹鲁卡品,对照组注射生理盐水,观察其行为学特征,并用RT-PCR方法检测大鼠海马齿状回内细胞因子IL-1 mRNA的动态表达变化。结果:大鼠腹腔注射锂-匹鲁卡品后30 min内相继出现严重癫痫持续状态发作,实验组各种细胞因子在严重癫痫持续发作后1 h表达水平开始明显增加(P<0.05),随着时间的延长表达逐渐增多,IL-1β的表达于发作后6 h达高峰,IL-1ra和IL-1R1则在发作后12 h达高峰,随后表达逐渐下降,各因子到发作后48 h表达降低但仍高于对照组表达水平(P<0.05)。结论:癫痫发作后急性期细胞因子IL-1β、IL-1ra及其受体IL-1R1在不同时间点均有增加,提示炎性因子参与了癫痫发作以及癫痫发作后脑损伤。AIM：To examine the expression of IL-1β,IL-1ra and IL-1R mRNA in the dentate gyrus of adult rats after lithium-pilocarpine（Li-PILO）-induced seizures.METHODS：Seizure epilepticus（SE） was induced using Li-PILO intraperitoneally（i.p.） injection.Lithium chloride was injected 18-20 h prior to PILO.The rats in the control group were given physiological saline instead of pilocarine.We observed the behavior of rats and examined the expression of cytokines mRNA in dentate gyrus by RT-PCR.RESULTS：Rats had seizure epilepticus in 30 min after administrated of pilocarpine.Cytokines（IL-1β,IL-1ra and IL-1R） began to increase significantly（P0.05） at 1 h after SE,and the peak was at 6-12 h.Expression of all cytokines declined on 48 h after SE.However these cytokines still kept higher than the control（P0.05）.CONCLUSION：Our findings suggestted that SE lead to increased expression of IL-1,IL-1ra and IL-1R1 after lithium-pilocarpine-induced seizures,Indicating that cytokines involved in SE and brain impairment after seizure.

关 键 词：癫痫 IL-1 mRNA 海马齿状回 

分 类 号：R741.02[医药卫生—神经病学与精神病学]