尿酸减轻6-羟基多巴胺对PC12细胞的毒性作用  

Protective Effect of Uric Acid on the Cell Damage of PC12 Cells Induced by 6-OHDA

作　　者：叶艳[1] 罗蔚锋[1] 朱婷鸽[1] 杨亚萍[1] 周旭平[1] 刘春风[1] 包仕尧[1] 

机构地区：[1]苏州大学附属第二医院神经内科,215004

出　　处：《中国临床神经科学》2010年第2期135-139,共5页Chinese Journal of Clinical Neurosciences

基　　金：苏州市科技计划(社会发展及医药)项目;江苏省高校自然研究计划项目(编号:08KJB320012)

摘　　要：目的:评价尿酸减轻6-羟基多巴胺(6-OHDA)对PC12细胞的毒性作用。方法:应用PC12细胞制作帕金森细胞模型,分为对照组、尿酸组、6-OHDA组、尿酸+6-OHDA组。采用MTT测定各组PC12细胞活性,免疫荧光法观察各组PC12细胞caspase-3激活情况,流式细胞术检测各组PC12细胞凋亡率。尿酸100～400μmol·L^(-1)不影响PC12细胞生存率,尿酸100～400μmol·L^(-1)可显著提高6-OHDA 50μmol·L^(-1)作用6、12和24 h造成的PC12细胞生存率的下降(P<0.01);尿酸能减少6-OHDA导致的PC12细胞caspase-3激活,降低6-OHDA导致的凋亡率(P<0.05)。结论:尿酸具有减轻6-OHDA对PC12细胞的毒性作用。Aim： To investigate the protective effect of uric acid（UA） on the injury of PC12 cells induced by 6-hydroxydopamine（6-OHDA）. Methods： PC12 cells injured by 6-OHDA were used as the Parkinson＇ s disease cell models, which were divided into four groups： control group, UA group, 6-OHDA group and UA＋6-OHDA group. The cell viability were assesseded by MTT method. The apoptosis of cells were assessed by observing the caspase-3 protein level by using immunocytochemistry, and calculating apoptosis rate using flow cytometry（FCM） technique. Results： 50 μmol·L-1 of 6-OHDA decreased the viability of PC12 cells at 6, 12, 24 h. 100-400μmol·L-1 of UA had no significant effect on the viability of PC12 cells. 100-400μmol·L-1 of UA reversed the decease of PC 12 cells induced by 6-OHDA. The caspase-3 protein level and apoptosis rate of PC12 cell were increased after 6-OHDA（50 ~tmol-L 1） treatment for 24 h, which were decreased by UA（200 μmol·L-1） treatment （P〈0.05）. Conclusion： 6-OHDA can decrease the viability of PC 12 cells and increase the apoptosis of PC12 cells. UA can protect PC12 cells from injury induced by 6-OHDA.

关 键 词：尿酸 帕金森病 PCI2细胞 6-羟基多巴胺 

分 类 号：R742.5[医药卫生—神经病学与精神病学]