运动训练对大鼠脑梗死灶周突触素mRNA及生长相关蛋白mRNA水平的影响  

Changes in synaptophysin and growth associated protein-43 mRNA around a cerebral infarction after mobility training

作　　者：谭来勋[1] 叶心国[1] 刘志超[1] 孙圣刚[2] 

机构地区：[1]武汉大学人民医院神经科,武汉430060 [2]华中科技大学同济医学院附属协和医院神经科

出　　处：《中华物理医学与康复杂志》2010年第3期166-168,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的研究运动训练对大鼠梗死灶周突触素mRNA和生长相关蛋白（GAP-43）mRNA的表达水平和大鼠运动功能的影响。方法将肾性高血压后的大脑中动脉闭塞脑梗死模型大鼠100只分成训练组和对照组（n=50），训练组大鼠进行运动训练，对照组大鼠常规饲养。2组大鼠均于制模成功后第3，7，14天采用横木行走实验评定各亚组大鼠运动功能，在制模成功后第1，3，7，14，28天时用半定量逆转录-多聚酶链式反应法检测各组大鼠脑梗死灶周边区突触素mRNA、GAP-43mRNA水平。结果造模后第7，14天，2组大鼠运动功能得分与本组造模后第3天比较，差异有统计学意义（P〈0．01）；制模后第7，14天时，训练组运动功能得分[分别为（3．2±0．3）分和（5．8±0．9）分]显著高于对照组[分别为（1．6±0．4）分和（2．6±0．8）分]，差异有统计学意义（P〈0．01）。2组大鼠脑梗死灶周突触素mRNA和GAP-43mRNA水平均在造模后第1，3，7天时逐渐增高，差异有统计学意义（P〈0．01）。训练组突触素mRNA水平在造模后第3，7，14和28天时均高于对照组，差异有统计学意义（P〈0．01）；训练组GAP-43mRNA水平仅在造模后第3和第7天时（0．295±0．03、0．512±0．045）高于对照组，差异有统计学意义（P〈0．01）。结论运动训练可使大鼠脑梗死灶周突触素mRNA和GAP-43mRNA水平增高，促进运动功能恢复。Objective To investigate the effects of mobility training on mobility and the mRNA levels of both synaptophysin and growth associated protein 43 （GAP-43） in the region around an infarction in rats with acute cerebral infarction. Methods Models of cerebral infarction were created in 100 rats through middle cerebral artery occlusion. They were then randomly divided into training and control groups. The motor skill of the rats was examined using a beam walking test. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction were observed at the 1 st, 3rd, 7th, 14th and 28th days after model-creation using a semi-quantitive reverse transcription polymerase chain reaction. Results The rats＇ mobility scores increased with training, and significant differences were observed between the average scores of the two groups at the 3rd, 7th and 14th days. The scores were higher in the training group. The mRNA levels of both synaptophysin and GAP-43 in the region around the infarction increased significantly from the 1st to the 3rd and 7th days. Synaptophysin mRNA levels were significantly higher in the trained group at each time：point, but the levels of GAP-43 mRNA were significantly higher in the trained group only on the 3rd and 7th days. Conclusions Motor skill and the mRNA levels of synaptophysin and GAP-d3 in the region around an infarction can be increased by motor skill training, at least in rats with model acute cerebral infarction.

关 键 词：突触素 生长相关蛋白 运动功能 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]