热放疗对舌鳞癌Tca8113细胞多药耐药蛋白及耐药性的影响  

The effect of thermo-radiotherapy on multidrug resistance in tongue squamous cell carcinoma cell line Tca 8113

作　　者：冷卫东[1] 罗志晓[1] 胡孝丽[1] 王大章[2] 冯戈[2] 

机构地区：[1]湖北省十堰市郧阳医学院附属太和医院口腔中心,十堰442000 [2]四川大学华硅口腔医学院颌面外科

出　　处：《中华物理医学与康复杂志》2010年第3期186-189,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金资助项目（39970798）,湖北省十堰市科技基金（2006s16）

摘　　要：目的探讨热放疗对舌鳞癌Tca8113细胞和耐药的Tca8113／CBDEA细胞耐药性的影响。方法应用免疫组化链霉菌抗生物素蛋白．过氧化物酶连结（SP）法检测热放疗对P-糖蛋白（P—gP）、多药耐药相关蛋白1（MRP1）和谷胱甘肽硫-转移酶π（GST-π）表达的影响和检测热放疗对细胞内阿霉素（ADM）浓度的影响。结果P—gP在Tca8113／CBDEA和Tca8113细胞中的表达在热放疗后第4小时和第24小时无明显变化（P〉0．05），MRP1在Tca8113／CBDEA中的表达无明显改变（P〉0．05），在Tca8113细胞中热放疗后第24小时MRP1有明显下降（P〈0．01）。在Tca8113／CBDEA和Tca8113细胞热放疗后第4小时GST-π无明显改变，第24小时均有明显下降。热放疗以后肿瘤细胞内ADM浓度有明显上升（P〈0．01）。结论热放疗联合应用能提高化疗的效果，抑制放疗所造成的多药耐药蛋白表达上升，提高细胞内的药物浓度，不会促使肿瘤细胞产生多药耐药现象（MDR）。Objective To study the effect of thermo-radiotherapy on multidrug resistance （MDR） and levels of intracellular adriamycin （ADM） in tongue squamous cell carcinoma cell line Tca 8113 and its MDR cell line Tca 8113/CBDEA. Methods Samples of the two cell lines were treated with thermo-radiotherapy （42℃ for 0. 5 h and 2 Gy of radiation）. Four and 24 hours later, the expression of the MDR relative proteins P-glyeoprotein （P-gp） , multidrug resistance associate protein 1 （MRP1） and glutathione s-tranferase-π （GST-π） were detected using immunohistochemistry, lntracellular ADM concentrations were measured using an HTS 7000 Plus bioassay reader. Results No change in the expression of P-gp was observed in the Tca 8113/CBDEA and Tca 8113 cell lines after 4 or 24 hours. Expression of MRP1 was not significantly altered in the Tca 8113/CBDEA cell line, but there was a significant drop in the Tca 8113 cell line 24 hours post-thermo-radiotherapy. Expression of GST-π was not altered in either the Tea 8113/CBDEA or the Tca 8113 cell line at 4 hours post-thermo-radiotherapy, but there was a significant decrease at 24 hours. At both 4 and 24 hours, drug tolerance had decreased and intracellular drug concentration had increased significantly in both cell lines. Conclusions Thermo-radiotherapy can enhance the effects of chemotherapy and suppress the expression of MDR factors induced by radiation. The combination of hyperthermia and radiotherapy does not induce MDR.

关 键 词：舌鳞状细胞癌 多药耐药 热放疗 

分 类 号：R739.8[医药卫生—肿瘤]