L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells  

作　　者：Hui XIE Bing YANG Xin-min ZHOU Feng-lin SONG Jian-ming LI Kang ZHOU Wen HU Yi-qun PENG Si-yuan TANG Ling-qing YUAN Si-yuan XIONG Xiao-bo LIAO 

机构地区：[1]Department of Cardiothoracic Surgery, the Second Xiang-Ya Hospital, Central South University, Changsha 410011, China [2]Institute of Metabolism and Endocrinology, the Second Xiang-Ya Hospital, Central South University, Changsha 410011, China [3]Department of Ultrasonic Diagnostics, the Second Xiang-Ya Hospital, Central South University, Changsha 410011, China [4]The School of Nursing of Central South University, Changsha 410013, China

出　　处：《Acta Pharmacologica Sinica》2010年第3期289-296,共8页中国药理学报（英文版）

基　　金：Acknowledgments This work was supported by the National Natural Science Foundation of China （30600661, 30872708, 30801174, 30871191 and 30900622）, the Scientific Research Foundation of the Health Bureau of Hunan Province （B2007058）, the Natural Science Foundation of Hunan Province （05C0163） and the Postdoctorate Scientific Research Special-purpose Project of Hunan Province （2008RS4012）.

摘　　要：Aim： To investigate the synergistic action of L-carnitine （LC） and taurine （TAU） on the proliferation and osteoblastic differentiation of vascular smooth muscle cells （VSMCs）. Methods： DNA and protein synthesis of VSMCs were assessed using scintillation counting. Alkaline phosphatase （ALP） activity and calcium content were determined to investigate the effects of LC and TAU on the osteoblastic differentiation and mineralization of VSMCs. TAU uptake by VSMCs was assayed. RNA interference was used to down-regulate the expression of the TAU transporter （TAUT） in rat VSMCs.Results： LC and TAU synergistically inhibited the proliferation and β-glycerophosphate （β-GP）-induced osteoblastic differentiation of VSMCs as evidenced by the decreased [^3H]thymidine incorporation, ALP activity and calcium deposition. Furthermore, LC stimulated the TAU uptake and TAUT expression in VSMCs. Suppression of TAUT with short hairpin RNA （shRNA） abolished the synergistic action of LC and TAU in VSMCs. Conclusion： The synergistic inhibitory action of LC and TAU on the proliferation and osteoblastic differentiation of VSMCs is attributable to the up-regulation of TAUT expression and TAU uptake by LC.Aim： To investigate the synergistic action of L-carnitine （LC） and taurine （TAU） on the proliferation and osteoblastic differentiation of vascular smooth muscle cells （VSMCs）. Methods： DNA and protein synthesis of VSMCs were assessed using scintillation counting. Alkaline phosphatase （ALP） activity and calcium content were determined to investigate the effects of LC and TAU on the osteoblastic differentiation and mineralization of VSMCs. TAU uptake by VSMCs was assayed. RNA interference was used to down-regulate the expression of the TAU transporter （TAUT） in rat VSMCs.Results： LC and TAU synergistically inhibited the proliferation and β-glycerophosphate （β-GP）-induced osteoblastic differentiation of VSMCs as evidenced by the decreased [^3H]thymidine incorporation, ALP activity and calcium deposition. Furthermore, LC stimulated the TAU uptake and TAUT expression in VSMCs. Suppression of TAUT with short hairpin RNA （shRNA） abolished the synergistic action of LC and TAU in VSMCs. Conclusion： The synergistic inhibitory action of LC and TAU on the proliferation and osteoblastic differentiation of VSMCs is attributable to the up-regulation of TAUT expression and TAU uptake by LC.

关 键 词：L-CARNITINE TAURINE vascular smooth muscle cells PROLIFERATION osteoblastic differentiation 

分 类 号：Q463[生物学—生理学] TB39[一般工业技术—材料科学与工程]