火木层孔菌菌丝体胞内色素提取与抗氧化活性初探  

Preliminary study on the extraction and antioxidant activity of endochrome of Phellinus igniarius

作　　者：何培新[1] 张迎春[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,郑州450002

出　　处：《食品科技》2010年第4期215-217,共3页Food Science and Technology

基　　金：中国科学院知识创新工程重要方向项目(KSCXZ-YM-N-46-02-04);郑州轻工业学院博士科研基金项目(000394)

摘　　要：火木层孔菌在中国传统上被称为桑黄,具有较强的抗癌等生理活性。在单因素实验的基础上,通过正交实验优化了火木层孔菌菌丝体胞内色素的提取工艺,并测定了粗提色素的抗氧化活性。结果表明,影响色素提取效果的因素主要是料液比,碱液提取的最佳条件为料液比1∶100、提取温度95℃、NaOH浓度2.5mol/L、提取时间75min,粗提色素得率较高(1.73%),并具有较强的抗氧化活性(对氧自由基的抑制率为91.68%)。该研究为桑黄真菌的综合开发应用提供了理论支持。Phellinus igniarius,also called Sanghuang in China,is one of the best medicinal fungus for its anti-tumor effect.Based on single-factor studies,orthogonal design was used to optimize the extraction technology of mycelial endochrome.The results suggested that among the tested factors,ratio of dosage liqueur was the main factor,and the best technology was：ratio of dosage liqueur 100,temperature 95 ℃,concentration of sodium hydroxide 2.5 mol/L and 75 minutes of extraction time.The extraction ratio was high（1.73%） and the crude endochrome exhibited strong antioxidant activity（91.68 %）.The research is very helpful to the utilization and exploitation of Sanghuang.

关 键 词：火木层孔菌(桑黄) 胞内色素 提取 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]