大孔吸附树脂法制备无患子皂苷及其抑菌活性表征  

Investigation on the Process of Sapindus Saponin Purified with Macroporous Adsorption Resin and Screening of Its Bacteriostasis

作　　者：傅勇[1] 雷鹏[1] 韩玉梅[1,2] 鄢丹[3] 

机构地区：[1]成都中医药大学,四川成都610075 [2]重庆理工大学,重庆400050 [3]解放军第302医院,北京100039

出　　处：《中药材》2010年第2期267-272,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:制备无患子皂苷抑菌活性部位。方法:以无患子皂苷的洗脱率、精制度为指标,考察大孔吸附树脂对无患子皂苷的吸附性能和洗脱参数,并采用管碟法评测各洗脱部位抑菌活性强弱,以确证无患子皂苷制备工艺的合理性。结果:13.6mL无患子皂苷样品液(生药0.01g/mL)上大孔吸附树脂柱(Φ15mm×H90mm,干重2.5g),用蒸馏水、30%乙醇、70%乙醇各3BV依次洗脱,抑菌活性部位无患子皂苷富集于70%乙醇洗脱液中。结论:通过大孔吸附树脂富集与纯化,无患子皂苷洗脱率为93.8%,精制度为250.1%,为无患子抑菌活性部位的开发提供了物质保障,亦为其它皂苷类化合物的纯化工艺研究提供了实验参考。Objective：To study the technological parameters of the purification process of saponins with macroporous adsorption resin. Methods： The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers. Bacteriostasis activity of each parts eluted was evaluated by the mean of cup-plate method. Results： 13.6 mL of the extraction of sapindus saponin （crude drugs 0.01 g/mL） was purified with a column of macroporous adsorption resin （Φ15 mm×H90 mm,dry weight 2.5 g） and washed with 3BV of distilled water, then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol, most of saponins were collected in the 70% ethanol. Conclusion： With macroporous adsorption resin adsorbing and purifying, the elutive ratio of saponins was 93.8% and the purity reached 250.1%. So this process of applying macroporous adsorption resin to adsorb and purify saponins is feasible, and supplies reference to the purification of other types of saponin.

关 键 词：大孔吸附树脂 纯化 无患子皂苷 抑菌活性 

分 类 号：R284.2[医药卫生—中药学]