Ras信号通路阻断剂FTI-277抑制HeLa细胞增殖及对cyclin E表达的影响  

Effects of a Ras signaling pathway inhibitor,FTI-277,on proliferation and cyclin E expression of HeLa cells

作　　者：孙迪[1] 沈宜[1] 汪少华[1] 向自武[1] 谢莹珊[1] 姜歆[1] 

机构地区：[1]重庆医科大学基础医学院病理生理教研室,干细胞与组织工程研究室,重庆400016

出　　处：《激光杂志》2010年第2期84-85,87,共3页Laser Journal

基　　金：重庆市渝中区科委基金资助项目

摘　　要：目的:观察Ras信号通路阻断剂(FTI-277)在人类宫颈癌细胞株HeLa细胞中对细胞周期素E(cyclin E)表达及对HeLa增殖、凋亡的影响情况。方法:采用MTT法观察不同浓度的FTI-277对HeLa细胞的生长抑制作用;流式细胞术(FCM)观察Hela细胞的细胞周期变化;RT-PCR、Western blot法检测FTI-277阻断Ras信号通路前后HeLa细胞cyclin E mRNA及蛋白表达。结果:各浓度范围FTI-277均能抑制HeLa细胞的增殖,且具有剂量依赖性和时间依赖性。流式细胞术分析显示,FTI-277作用后的HeLa细胞明显滞留于G1/S期,且具有时间依赖性。RT-PCR及Western blot结果显示,FTI-277作用前后HeLa细胞cyclin E mRNA表达无明显变化,而蛋白表达水平明显降低。结论:FTI-277可以抑制细胞增殖,Ras信号转导通路可能是通过影响HeLa细胞cyclin E转录后的翻译环节减少其表达。AIM： To observe the effects of FTI-277,a Ras signaling pathway inhibitor,on the expression of cyclin E and on the proliferation and apoptosis of human Cervical cancer cell lines（HeLa cells）.Methods：HeLa cells were treated with different concentrations of FTI-277,MTT assay was used to observe the proliferation of HeLa cells,flow cytometry（FCM） was used to examine the growth cycles of the HeLa cells.The mRNA and protein expression of cyclin E were detected by RT-PCR and Western blot before and after Ras signal was blocked by FTI-277 a Ras inhibitor.RESULTS：The concentration range of FTI-277 inhibited the proliferation of HeLa cells in a concentration-and time-dependent manner.Flow cytometry showed that FTI277 blocked HeLa cells in G1/S phase in a time dependent way.RT-PCR and Western blot exhibited that the cyclin E mRNA expression in HeLa cells had no significant change,but significantly reduced the level of protein expression before and after HeLa cells were treated with FTI-277.Conclusion：FTI-277 could inhibit HeLa cells proliferation.Ras signal transduction pathway may influence the translation of cyclin E post-transcriptional to reduce its expression in HeLa cells.

关 键 词：FTI-277 宫颈癌细胞株(HeLa细胞) CYCLINE RAS 

分 类 号：R477[医药卫生—护理学]