小檗碱治疗腹泻的分子机制研究  

The anti-diarrhea mechanisms of berberine in diarrhea disease

作　　者：张永国[1] 王新[1] 刘霖[1] 柴娜[1] 李泉江[1] 吴开春[1] 

机构地区：[1]第四军医大学西京消化病医院,肿瘤生物学国家重点实验室,西安710032

出　　处：《陕西医学杂志》2010年第1期6-8,共3页Shaanxi Medical Journal

摘　　要：目的:探讨小檗碱治疗腹泻的可能分子机制。方法:利用番泻苷A诱导建立BALB/c小鼠的腹泻模型,给予小檗碱治疗处理,采用半定量RT-PCR检测影响肠管钠水吸收的钠-氢交换体1(NHE1)和钠-氢交换体3(NHE3)的表达情况;利用免疫组织化学的方法,作进一步证实。结果:半定量RT-PCR表明小檗碱处理组NHE3mRNA的表达水平较腹泻组小鼠明显增高,但与正常对照组相比,无显著性差异;而NHE1mRNA的表达水平在三组间并无明显差异。免疫组化的结果也证实,与正常对照组和处理组小鼠相比,腹泻组小鼠NHE3的表达水平明显下调。结论:小檗碱可通过上调NHE3mRNA和蛋白的表达而不是NHE1的表达,进而增强肠管对钠水的吸收,从而发挥治疗腹泻的作用。Objective：To explore the molecular mechanisms by which berberine might exhibit its anti-diarrhea effect. Methods：Diarrhea mice model were induced by sennosideaA,and then treated with berberine. The expression of NHE1 and NHE3 mRNA which affect the absorption of Na＋ and water was detected by semi RT-PCR.Furthermore,immunohistochemistry was adopted to confirm the results acquired by RT-PCR. Results：Compared with treatment group and control group,the expression of NHE3 were detected by semi RT-PCR and immunohistochemistry were significantly decreased in diarrhea mice induced by sennosideA,but the expression of NHE1 were no statistical significance among there groups. Conclusion：Berberine can increase the expression of NHE3 not NHE1,suggesting berberine might exhibit its anti-diarrhea effect partially by enhancing the absorption of Na＋ and water.

关 键 词：腹泻/药物疗法 生物碱类/治疗应用 免疫组织化学 模型 动物 小鼠 

分 类 号：R574.4[医药卫生—消化系统]