水通道AQP2在多囊肾上皮中的表达及意义  

Expression of aquaporin-2 in ranal cyst epithelial in polycystic kidney disease

作　　者：杨龙飞[1] 朱娜[1] 宗敏茹[2] 何成彦[2] 冯学超[1] 

机构地区：[1]东北师范大学膜通道实验室,吉林长春130024 [2]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国实验诊断学》2010年第4期490-491,共2页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30770493)

摘　　要：目的探讨水通道蛋白AQP2在人多囊肾囊肿上皮中的表达并探讨其意义。方法利用RT-PCR及免疫组织化学等方法对人多囊肾囊泡上皮表达水通道AQP2情况进行初步分析。结果AQP2在人多囊肾囊肿上皮表达程度较高,且在上皮细胞近囊腔侧膜的表达水平明显高于胞浆。结论AQP2可能参与人多囊肾的形成过程。深入研究AQP2在多囊肾囊肿上皮中的作用,将为多囊肾疾病的分子成因提供新的机理。Objective To explore the expression of AQP2 in ranal cyst epithelial of polyeystie kidney.Methods Assay the expression of AQP2 in ranal cyst epithelial of polycystic kidney, by RT-PCR and immunohistochemistry. Results AQP2 were found highly expressed in ranal cyst epithelial of polycystic kidney, the expresion level of AQP2 located in intracellular vesicles is higher than that in the apical plasma membrane. Conclusion AQP2 may play an important role in formation of polycystic kidney disease. Further investigation of AQP2 function in ranal cyst epithelial of polycystic kidney may identify new molecular mechanisms of genetic polycystic kidney disease.

关 键 词：水通道 多囊肾疾病 囊肿上皮 RT-PCR 免疫组化 

分 类 号：R692.1[医药卫生—泌尿科学]