Neuregulin-1β对大鼠缺血再灌注后GAP-43表达及神经功能的影响  

Effects of Neuregulin-1β on GAP-43 expression and neurobihavioral improvement after cerebral ischemic reperfusion in rats

作　　者：蔡东妙[1] 张良成[1] 周琳瑛[2] 郑晓燕[1] 赖忠盟[1] 何莹[3] 

机构地区：[1]福建医科大学附属协和医院麻醉科,福州350005 [2]福建医科大学基础医学院病理学系电镜室 [3]福建医科大学附属第三医院

出　　处：《中国实用医药》2010年第11期1-3,共3页China Practical Medicine

基　　金：福建省科技厅三项费基金资助(项目编号:2007F5054)

摘　　要：目的观察神经调节蛋白-1(Neuregulin-1β;NRG-1β)对大鼠脑缺血再灌注后灰质梗死周围区生长相关蛋白(GAP-43)的影响。方法成年健康雄性SD大鼠54只,随机分NRG-1β给药组,NS对照组,空白对照组。采用线栓法建立大脑中动脉缺血再灌注动物模型(MCAO/R),分别于MCAO/R后1 d组,3 d组和7 d组进行神经功能学评分;斜板实验:测大鼠随意运动功能及RT-PCR法观察GAP-43 mRNA的表达。结果NRG-1β组3 d和7 d,GAP-43 mRNA表达较NS对照组和空白组表达增加,差异有统计学意义(P<0.05)。NRG-1β注射后7 d,神经功能也有改善。结论神经调节素促进大鼠脑灰质缺血再灌注后神经功能恢复,改善大鼠随意运动和提高GAP-43 mRNA表达,具有神经保护作用。Objective To study the neuroprotective effects of neuregulin-1β（NRG-1β）after cerebral ischemic reperfusion in rats.Methods 54 adult male rats were divided randomly into NRG-1β group,NS group and sham group.Nylon Monofilament was applied to establish the middle cerebral artery occlusion reperfusion models（MCAO/R）in the rats.The rats in each group were sacrificed at 1 d,3 d and 7 d after MCAO/R.The nervous behavioral function was evaluated with Zea longa score and inclined plate experiment after the rat wake one hour.RT-PCR was used to detect the expression of GAP-43 mRNA in in the gray matter around the cerebral infarct of rats.Results The expression of GAP-43 mRNA in gray matter of rats were obviously increased in NRG-1β group compared with controlled group and sham group（P〈0.05）.Also the nervous behavioral function had better restoration in NRG-1β group.Conclusion Neuregulin-1β may have neuroprotection by improving the nervous behavioral founction and the expression of GAP-43 mRNA in gray matter around the cerebral infarct of rats.

关 键 词：神经调节蛋白-1 GAP-43蛋白 脑缺血 再灌注 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]