鬼臼毒素衍生物Z-26-2诱导K562/A02细胞凋亡实验研究  

Study on apoptosis in K562/A02 cell line induced by derivative of podophyllotoxin Z-26-2

作　　者：高洁[1] 吕晶晶[1] 张元[1] 陈虹[1] 

机构地区：[1]武警医学院生药学教研室,天津300162

出　　处：《武警医学院学报》2010年第4期253-255,F0002,共4页Acta Academiae Medicinae CPAPF

基　　金：国家自然科学基金资助项目(30873363)

摘　　要：【目的】研究鬼臼毒素衍生物Z-26-2对多药耐药肿瘤细胞株K562/A02的促凋亡作用。【方法】体外培养K562/A02细胞,经Z-26-2作用后,应用SRB法测定GI50及生长曲线;Giemsa染色、荧光染色、DNA ladder、流式细胞术观察Z-26-2诱导K562/A02细胞凋亡作用。【结果】Z-26-2对K562/A02细胞促凋亡作用显著,呈现典型的"DNAladder",流式细胞术发现经不同浓度的Z-26-2(1μm、2μm)作用K562/A02细胞12h、24h后,出现明显的凋亡峰,且凋亡细胞的比例随着Z-26-2浓度的升高而增加,呈一定的剂量依赖性,并可将细胞周期阻断在S期。【结论】Z-26-2通过调控细胞周期驱动机制使细胞发生周期阻滞,对多药耐药肿瘤细胞k562/A02具有明显抑制作用。[Objective]To study the apoptosis and mechanism in cell line K562/A02 induced by Z-26-2,a derivative of podophyllotoxin. [Methods] SRB assay,Giemsa stain,Hoechst33342 stain,DNA ladder ,and cell cycle ananlysis were used to examine the apoptosis in K562/A02 cells. [Results] The higher the concentration of Z-26-2,the clearer the morphous of apoptosis. And it showed a significant dose dependence. K562/A02 were exposed to Z-26-2 for 24 h,and we found DNA ladder was very obvious. Rates of apoptosis afer 12 and 24 h were checked through flow cytometry,and it showed that the apoptotic cells caused by Z-26-2 （1μm、2μm） was dose-dependent. Cell cycle analysis indicated that Z-26-2 blocked the cell cycle at S phase.[Conclusions] Z-26-2 showed obvious anticancer activity by causing cell cycle arrest through regulating the cell cycle.

关 键 词：鬼臼毒素衍生物 细胞周期 细胞凋亡 K562/A0 

分 类 号：R282.71[医药卫生—中药学]