植物中药IHA-01抑制人肺癌GLC细胞增殖和诱导凋亡的实验研究  

An experimental study of plant traditional Chinese medicine IHA-01 suppressing lung neoplasms GLC cell multiplication and inducing its apoptosis

作　　者：王苗[1] 董坚[1] 李秋恬[1] 吕加令[1] 武治国[1] 毛剑锋[1] 

机构地区：[1]昆明医学院第一附属医院生物治疗中心,650032

出　　处：《中国医药生物技术》2010年第2期105-109,共5页Chinese Medicinal Biotechnology

基　　金：云南省应用基础研究计划(2007C0008R)

摘　　要：目的初步探讨植物中药IHA-01抑制人肺癌GLC细胞增殖及诱导其早期凋亡的作用。方法人肺癌GLC细胞经不同浓度(0.01、0.1、1、10、100μg/ml)IHA-01作用24、48、72h,以MTT法进行IHA-01有效作用浓度筛选并确定IHA-01工作浓度(1/2IC50);透射电镜观察IHA-01细胞超微结构;流式细胞术进行DNA倍体分析和TUNEL分析,检测IHA-01对肺癌GLC细胞周期及早期凋亡的影响。结果IHA-01工作浓度(1/2IC50)为0.7μg/ml,且不同浓度IHA-01作用不同时间对GLC细胞增殖的抑制作用呈时效和量效依赖关系;透射电镜发现IHA-01实验组部分细胞出现细胞连接消失,微绒毛消失,胞质密度增加,可见细胞早期凋亡改变,具有诱导细胞分化和早期凋亡的作用;流式细胞术显示肺癌GLC细胞出现明显的凋亡峰,细胞周期阻滞在G0/G1期,S期细胞减少;流式细胞术TUNEL法检测结果显示,细胞凋亡和坏死同时存在。结论IHA-01具有抑制肺癌GLC细胞增殖,诱导凋亡的作用,具有深入研究的价值。Objective To explore the proliferation-inhibiting and the early apoptosis-inducing activity of traditional Chinese medicine IHA-01 on human lung neoplasms cell line GLC.Methods After being treated with different concentrations of HIA-01（0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml and 100μg/ml, respectively）for 24,48 and 72 hours,the effective working concentration and its working concentration（1/2 IC50）were detected by MTT.Observed the cell ultrastructure in each group by TEM.Apply the FCM DNA assay and TUNEL assay to examines the influence of IHA-01 which induce the lung neoplasms GLC mitotic cycle and the early apoptosis process. Results IHA-01 work concentration（1/2 IC50）was 0.7μg/ml,the inhibition effect of IHA-01 on the GLC cell growth showed time-effect dependence and dose-effect dependence.The results of TEM found that some cells＇connection disappeared and microvilli disappeared,cytoplasm density increased,and the change of cell nuclear and early apoptosis was appeared in dealing with IHA-01.FCM assay indicated that most of the cells were arrested in G0/G1 and the apoptotic peak appeared and the number of S phase cells decreased.The results of TUNEL indicated that there were apoptosis and necrosis simultaneous. Conclusion IHA-01 can inhibit lung neoplasms cell proliferation and induce apoptosis in vitro,and has the deep research value.

关 键 词：中草药 肺肿瘤 细胞增殖 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]