西南山茶花化学成分的定量分析  

Determination of Chemical Constituents in Camellia Pitardii

作　　者：侯蕾[1] 唐玲[1] 史丽颖[1] 冯宝民[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622

出　　处：《云南中医学院学报》2010年第2期25-28,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(NO:30772714)

摘　　要：目的:旨在测定西南山茶花醇提物、花的醇提物乙酸乙酯萃取物、水层中总皂苷、总多酚、总黄酮的含量。方法:采用紫外分光光度法。结果:其总皂苷、总多酚、总黄酮的含量依次为:西南山茶花醇提物12.15%,32.4%,3.27%;花的醇提物乙酸乙酯萃取物25.86%,37.0%,8.64%;水层5.78%,12.76%,0.34%.结论:紫外分光光度法测定以上三种物质简便易行,准确可靠,重现性较理想,回收率较好。OBJECTIVE： To determine the chemical constituents of total saponin, total polyphenol （tannin） and total flavonoids in section Camellia pitardii. METHOD： Their contents were determined by spectrophotometry. RESULTS ： The contents of total saponin, total polyphenol （tannin） and total flavonoids were respectively determined as： alcohol extract of Camellia pitardii 12. 15%, 3.27%, 32. 4% ; ethyl acetate extracts of Camellia pitardii 25.86%, 37.0%, 8.64% ; water layer 5.78%, 12.76%, 0. 34%. CONCLUSIONS： The method is convenient and reliable to determinate the three substance , the reproducibility and the recovery were fairly well.

关 键 词：西南山茶花 总皂苷 总多酚 总黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学]