沙土鼠全脑缺血再灌注损伤的实验研究  

Experimental Study on the Cerebral Damage Caused by Interrupted,Repeated Arterial Occlusion Versus Uninterrupted Occlusion in Gerbil Forebrain Ischcmic Model

作　　者：梁勇[1] 周良辅[1] 毛颖[1] 张峰[1] 郭欢欢[1] 

机构地区：[1]上海医科大学附属华山医院神经外科,200040

出　　处：《中国临床神经科学》1998年第2期72-75,共4页Chinese Journal of Clinical Neurosciences

摘　　要：比较暂时单次阻断和分次阻断动脉所致脑缺血损伤的严重程度.24只雄性沙土鼠经双侧颈总动脉阻断脑缺血模型,分单次阻断10min组与阻断5min,灌注10min,再阻断5min.24h后断头取脑行抗微管相关蛋白2(MAP2)免疫组化染色,计算机图像分析系统行病变范围测定.分次阻断组动物的海马下脚CA1区及额顶叶脑皮层Ⅲ～Ⅳ层缺血性损伤程度比单次长时间阻断减少了2.04%,但两者比较无明显的统计学差异(P>0.05).分次阻断间歇期行再灌注能否加重脑缺血损伤的程度主要受分次阻断的强度、阻断持续时间、再灌注时间的长短以及个体的脑血管侧支循环能力而定.To study the extent of ischemic injury caused by interrupted ,repeated arterial occlusion versus uninterrupeted occlusion using gerbil forebrain ischemic model. 24 Mongolian male gerbils were delivered to sham-operated control group, uninterrupted occlusion group (10 minutes temporary bilateral CCA occlusion) , interrupted occlusion groups (one separate 5-minute period of occlusion, with 10 minutes of reperfusion between occlusions, respectively). The animals were sacrificed 24 hours later after the initial arterial occulusion. Immunohistochemical evaluation for ischemic neuronal damages was conducted. The computer image analysis system was used to measure the areas of ischemic neuronal damage. Loss of immunohistochemical evaluation for microtube associated protein 2(MAP2) in the dendrites of neurons became apparent in the subiculum-CA1 region and in the layer III / IV of the cerebral cortex in the experimental groups. The animals in the group with interrupted, repeated occlusion showed a 2. 04% decrease in the areas of ischemic neuronal damages compared with the uninterrupted occlusion group. The benefit of interrupted arterial occlusion against neuronal is- chemic damage did not quite reach statistical significance (P> 0. 05). The results suggest that intermittent reperfusion may have differential effects mainly depending on the residual

关 键 词：脑缺血 暂时动脉阻断 再灌注损伤 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]