水通道蛋白5在人胎肺发育中的表达及意义  

Expression and significance of aquaporin 5 in human fetal lung development

作　　者：牛惠惠[1] 曾万江[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国优生与遗传杂志》2010年第4期75-76,25,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的检测水通道蛋白5(AQP5)在胎儿正常肺组织和发育异常肺组织的表达,探讨AQP5对胎肺发育的影响及其在肺液代谢中的作用。方法应用RT-PCR检测AQP5mRNA在各胎肺中的表达以及real-timePCR检测AQP5在正常胎肺和发育异常胎肺中的基因表达差异。结果AQP5mRNA在各孕周胎肺均有表达,发育异常胎肺组织较正常胎肺组织表达量明显减少,差异有统计学意义(P<0.05))。结论发育异常胎肺AQP5表达下降,其对胎肺的正常发育和肺液代谢可能有重要影响。Objective：To detect the expression of aquaporin 5 （AQP5） in normal and abnormal human fetal lungs and to discuss the role of AQP5 in fetal lung development and pulmonary fluid metabolism.Methods：The expression of AQP5 mRNA in human fetal lung was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）.The expression difference of AQP5 mRNA was detected by semi-quantitative real-time PCR.Results：AQP5 mRNA was detected in the fetal lungs from normal and abnormal lungs.Expression level of AQP5 was significantly reduced in abnormal fetal lungs compared to normal ones（P 0.05）.Conclusion：AQP5 shows decreased expression in abnormal fetal lungs.AQP5 may have a significant impact on normal lung development and pulmonary fluid metabolism.

关 键 词：水通道蛋白5 胎肺发育 肺泡Ⅰ型上皮细胞 

分 类 号：R321.54[医药卫生—人体解剖和组织胚胎学]