细针抽吸活组织检查与多聚合酶链反应——顺序特异性引物协同诊断胰腺癌  

Application of Fine Needle Aspiration Biopsy and Polymerase Chain Reaction-Sequence Special Primers in the Diagnosis of Pancreatic Adenocarcinoma

作　　者：戴存才[1] 杜竞辉[1] 刘训良[1] 苗毅[1] 张兆松[2] 苏川[2] 

机构地区：[1]南京医科大学第一附属医院普外科,210029 [2]南京医科大学分子生物学研究所,210029

出　　处：《外科理论与实践》1998年第2期98-99,107,共3页Journal of Surgery Concepts & Practice

摘　　要：目的:探索细针抽吸活组织检查(FNAB)和PCR-SSP协同检测对胰腺良恶性肿块鉴别及对胰腺癌的诊断价值.方法:对35例胰腺癌、12例正常胰腺组织、26例胰腺良性疾病、8例壶腹癌及7例胆管癌行FNAB,并对FNAB吸出物行PCR-SSP检测K-ras基因有无突变.结果:FNAB对胰腺癌的确诊率为57.1%(20/35),对壶腹癌及胆管癌的确诊率分别为4/8(50)和4/7(5.1%);PCR-SSP对胰腺癌的确诊率为91.4%(32/35),对壶腹癌及胆管癌的确诊率皆为零.结论:FNAB与PCR-SSP协同检测有助于确诊胰腺癌;PCR-SSP对壶腹癌及胆管癌的诊断价值不大.Objective: To assess the value of FNAB together with PCR-SSP in the differentiation of pancreatic benign and malignant nodules, and in the definitive diagnosis of pancreatic adenocarcinoma. Methods: FNAB was carried out in 35 cases of pancreatic carcinoma, 12 cases of normal pancreatic tissue, 26 cases with benign pancreatic diseases, 8 cases of ampullary carcinoma and 7 cases of bile duct carcinoma; also, the aspirated material underwent detection for K-ras gene mutations by PCR-SSP. Results: In theFNAB group, the correct diagnosis rate (CDR) of pancreatic carcinoma was 57.1%(20/35), that of ampullary carcinoma and bile duct carcinoma were 4/8(50%) 和 4/7(57.1%), respectively. In the FNAB associated with PCR-SSP group, the CDR of pancreatic carcinoma was 91.4%(32/35), but that for both ampullary carcinoma and bile duct carcinoma were zero. Conclusions: FNAB combined with PCR-SSP yield a higher accurate diagnosis rate for pancreatic adenocarcinoma, while PCR-SSP is not useful for the diagnosis of ampullary and bile duct carcinoma.

关 键 词：胰腺癌 PCR-SSP 细针抽吸 活组织检查 诊断 

分 类 号：R735.904[医药卫生—肿瘤] R730.43[医药卫生—临床医学]