人卵巢癌细胞HABP1的表达及其与侵袭转移能力的相关性  

Expression of HABP1 and its relationship with the invasion and migratory ability in human ovarian cancer cells

作　　者：雷利娜[1] 薛秀珍[1] 王晶[2] 

机构地区：[1]河南科技大学第一附属医院,河南洛阳471003 [2]哈尔滨医科大学附属肿瘤医院

出　　处：《山东医药》2010年第15期13-15,共3页Shandong Medical Journal

基　　金：黑龙江省教育厅科技项目(10541113)

摘　　要：目的探讨透明质酸结合蛋白1(HABP1)在卵巢癌侵袭转移过程中的作用。方法分别用荧光实时定量RT-PCR和Western blot法检测HABP1 mRNA和HABP1蛋白在人卵巢癌细胞株HO-8910及高转移HO-8910PM细胞株中的表达,采用体外侵袭实验测定细胞侵袭能力。结果HO-8910PM中HABP1 mRNA和蛋白的表达水平均高于HO-8910,HO-8910PM的侵袭转移能力高于HO-8910。结论HABP1的高表达可能在人卵巢癌细胞的侵袭转移过程中发挥一定作用。Objective To investigate the effects of hyaluronan binding protein 1 （HABP1） in the invasion and migration of ovarian cancer. Methods The fluorescent quantitation RT-PCR and Western blot methods were used to examine the expression of HABPI mRNA and HABP1 protein in ovarian cancer cell lines HO-8910 and highly metastatic HO-8910PM, the invasion and migratory ability of the two types of cell were observed by invasion assay in vivo. Results The levels of HABP1 mRNA and protein in HO-8910PM cells were both higher than those in HO-8910 cells,the invasion and migration capability of HO-8910PM cells was higher than that of HO-8910 cells. Conclusion The high expression of HABP1 may play certain role in the invasion and migration of human ovarian cancer ceils.

关 键 词：人卵巢癌细胞 透明质酸结合蛋白1 侵袭 转移 

分 类 号：R737.31[医药卫生—肿瘤]