PHⅡ-7的体内外抗肿瘤活性及机制  

Antitumor activity and mechanism of PH Ⅱ-7 in vitro and in vivo

作　　者：李洪强[1] 程昕[1] 周园[1] 胡蕴慧[1] 韩勇[1] 李巍[1] 杨纯正[1] 高瀛岱[1] 

机构地区：[1]中国医学科学院北京协和医学院血液病医院血液学研究所实验血液学国家重点实验室,天津300020

出　　处：《山东医药》2010年第15期21-23,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30873091;30971291)

摘　　要：目的研究PHⅡ-7的体内外抗肿瘤活性,为肿瘤的治疗提供新思路。方法采用MTT法检测PHⅡ-7对18种细胞系(其中包括5对敏感肿瘤细胞和相应耐药细胞株)的体外抗肿瘤活性;建立人白血病细胞K562和K562/A02裸鼠移植瘤模型,研究PHⅡ-7的体内抗肿瘤活性。结果PHⅡ-7对人肿瘤细胞的生长均有抑制作用,IC50为0.35～18.68μmol/L;其对于多药耐药(MDR)类细胞亦显示出较强的抑制作用。PHⅡ-7对耐药肿瘤细胞(K562/A02)移植瘤模型的抑制率为62%,对敏感肿瘤细胞(K562)移植瘤模型的抑制率为52%。结论PHⅡ-7体内外均可抑制肿瘤细胞的生长,其抗耐药肿瘤的作用可能存在多种机制。本研究为肿瘤的治疗提供了新思路。Objective To investigate the antitumor activity of PH Ⅱ -7 in vitro and in vivo , so as to provide new thinking for the treatment of tumor. Methods MTT was used to test the antitumor activity of PH Ⅱ -7 against 18 human tumor cells lines（ including 5 pairs of MDR sublines and their parental cell lines） in vitro; The in vivo efficacy of PH Ⅱ-7 was evaluated using xenografts of human leukemic K562 cells and K562/A02 by implantation in BULB/e nude mice. Results PH Ⅱ -7 inhibited the proliferation of various human tumor cells, the IC50 ranged from 0.35-18.68 μmol/L. Especially, PH Ⅱ-7 had the same antitumor effect on MDR tumor cells in vitro. PH Ⅱ-7 inhibited the tumor growth by 62% and 52% respectively in the K562/AO2 and K562 implantation models. Conclusions PH Ⅱ -7 can inhibit the growth of tumor cells in vitro and in vivo, its effects against MDR tumor have multiple mechanism. This study provides new thinking for the treatment of tumor.

关 键 词：多药耐药 氧化吲哚衍生物 小分子化合物 

分 类 号：R730.1[医药卫生—肿瘤]