乳腺癌和非癌组织中DLC1基因及蛋白表达与Ki-67的关系  

Expression of DLC1 mRNA and protein in breast carcinoma and non-cancerous breast tissues and its relationship with Ki-67

作　　者：刘宇飞[1] 张兆祥[1] 淡汉进[2] 陈路[3] 黄益玲[1] 高艳[3] 韩林[1] 

机构地区：[1]三峡大学医学院病理学教研室,宜昌443002 [2]湖北省枝江市人民医院病理科,枝江443200 [3]三峡大学第一临床医学院病理科,宜昌443003

出　　处：《临床与实验病理学杂志》2010年第1期27-31,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨乳腺癌和非癌组织中DLC1表达及其与Ki-67的相互关系。方法应用原位杂交技术和免疫组织化学En-Vision两步法,检测52例乳腺浸润性导管癌和42例非癌组织(包括癌旁乳腺组织20例和乳腺纤维腺瘤22例)中DLC1-mR-NA、DLC1和Ki-67的表达。结果DLC1-mRNA和蛋白在乳腺癌中的阳性率(50%和57.7%)低于非癌乳腺组织(90.5%和92.9%),差异有统计学意义(χ2=17.518和10.729,P<0.01)。DLC1-mRNA与蛋白的表达呈正相关(rs=0.379,P<0.01)。Ki-67在乳腺癌中的阳性率为61.5%,而在非癌组织中均呈阴性表达,两者差异有显著统计学意义(χ2=39.186,P<0.01)。乳腺癌中DLC1与Ki-67的表达呈负相关(rs=-0.507,P<0.01)。结论DLC1-mRNA和蛋白在乳腺癌中呈低表达或缺失,提示其与乳腺癌的发生、发展有关,DLC1有抑制乳腺癌细胞增殖的功能,可作为乳腺癌新的分子标记物。联合检测DLC1和Ki-67在乳腺癌中的表达,有望成为估价乳腺癌生物学行为的参考指标。Purpose To investigate the expression of DLC1 and its relationship with Ki-67 in cancerous and non-cancerous tissues of the breast.Methods In situ hybridization and immunohistochemiscal EnVision method were used to detect the expression of DLC1 mRNA and protein and Ki-67 in 52 invasive breast ductal carcinomas and 42 non-cancerous mammary tissues,including 22 mammary fibroadenomas and 20 paracancerous tissues.Results The positive rates of DLC1 mRNA and protein expression in the breast carcinomas （50% and 57.7%） was significantly lower than that in the non-cancerous mammary tissues （90.5% and 92.9%） （χ^2=17.518 and 10.729,P〈0.01）.The expression of DLC1-mRNA was positively related to DLC1protein （rs=0.379,P〈0.01）.The positive rate of Ki-67 expression was 61.5% in the breast carcinomas,but no expression was observed in the all non-cancerous tissues （χ^2=39.186,P〈0.01）.Correlation analysis showed that DLC1 expression was negatively correlated with Ki-67 expression （rs=-0.507,P〈0.01）.Conclusions Lower or no expression of DLC1 mRNA and protein may play an important role in the pathogenesis and progression in breast carcinoma.DLC1 may inhibit the proliferation of the breast carcinoma cells,which indicates that it may act as a new molecular marker of breast carcinoma.Combining detection of DLC1 and Ki-67 may be useful parameters for evaluating the biological behaviors of breast carcinoma.

关 键 词：乳腺肿瘤 DLC1 KI-67 免疫组织化学 原位杂交 

分 类 号：R737.9[医药卫生—肿瘤] R341[医药卫生—临床医学]