栀子粗提物对拟痴呆模型鼠学习记忆功能障碍的影响  

Effects of Crude Extract of Cape Jasmine on Learning and Memory Function in Experimental Dementia Animal Model

作　　者：杨楠[1] 刘雁勇[1] 郝文宇[1] 纪超[1] 梁妍[1] 朱海波[2] 左萍萍[1] 

机构地区：[1]中国医学科学院基础医学研究所药理室,北京市100005 [2]中国医学科学院药物研究所,北京市100050

出　　处：《中国康复理论与实践》2010年第4期308-310,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：科技部重大新药创制科技专项项目(2009ZX09303)

摘　　要：目的观察中药栀子粗提物对Aβ25-35致痴呆小鼠模型及鹅膏蕈氨酸(IBO)致大鼠记忆获得障碍模型的影响。方法采用Aβ25-35脑室注射致痴呆小鼠模型及前脑基底核注射IBO致大鼠记忆获得障碍模型,通过Morris水迷宫实验和避暗实验,观察栀子粗提物低、中、高3个剂量组(12.5、25、50mg/kg)对模型鼠学习记忆功能的影响。阳性药采用多奈派齐(0.75mg/kg)。结果栀子粗提物3个剂量均可提高Aβ25-35致痴呆小鼠的Morris水迷宫空间学习记忆能力(P<0.05),中剂量对IBO致记忆获得障碍大鼠水迷宫空间学习记忆能力有明显改善作用(P<0.01);各给药组大鼠受到避暗箱内电击的潜伏期均有所延长,3min内受到电击的次数均有所减少。结论栀子粗提物有改善拟痴呆模型鼠学习记忆功能的作用。Objective To assess the effects of crude extract of Cape Jasmine in the experimental Alzheimer＇s model induced by Aβ25-35 and the memory acquisition impaied model induced by ibotenic acid（IBO）.Methods Alzheimer＇s dementia of mice was induced by Aβ25-35 i.cv. treatment and the impaired memory acquisition model was induced by IBO injected into the forebrain nucleus basalis. The effects of crude extract of Cape Jasmine on the learning and memory function of model animals were evaluated with Morris water maze and step-through test in 3 dose groups（12.5,25,50 mg/kg）. Donepezil（0.75 mg/kg）was used in the positive control group.Results Morris water maze test showed that the spatial learning and memory ability of the model mice significantly improved in three crude extract of Cape Jasmine groups（P〈0.05）. Meanwhile,the impaired function of the rats induced by IBO significantly improved in the medium dose group（P〈0.01）. The electric shock latent period in step-through box test prolonged and the frequency of electric shock decreased within 3 minutes in three groups.Conclusion Crude extract of Cape Jasmine can improve the learning and memory function of mice or rat in the model group.

关 键 词：栀子 粗提物 拟痴呆动物模型 学习记忆 MORRIS水迷宫 避暗实验 

分 类 号：R742[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]