非小细胞肺癌患者外周血细胞化疗药物敏感性及其临床意义的研究  

Study of chemosensitivty and clinical significance of peripheral blood lymphocytes and granilu vytes in non-small-cell lung cancer patients

作　　者：唐东平[1] 秦婷婷[1] 苏承武[2] 李永强[1] 胡晓桦[1] 朱波[1] 黄玲莎[1] 

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,广西南宁530021 [2]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《现代肿瘤医学》2010年第4期697-699,共3页Journal of Modern Oncology

基　　金：广西自然科学基金(编号:桂科基0575091)

摘　　要：目的:通过非小细胞肺癌外周血淋巴细胞和粒细胞体外培养,对其常用的化疗药物进行敏感性检测,以筛选出最佳的化疗方案。方法:应用MTT吸光度法测定32例非小细胞肺癌患者外周血标本对临床常用的4种化疗药物及其组成的2个联合化疗方案的敏感性或耐药性,比较其差异。结果:非小细胞肺癌化疗敏感性个体差异较大,联合化疗药物的敏感性明显优于单药。各药物对淋巴细胞和粒细胞的平均抑制率由高到低的顺序一致,各用药方案间比较均存在差异,部分有显著性差异(P<0.01)。结论:外周血有核细胞体外药敏试验或耐药试验对临床肿瘤化疗用药有一定的预见性和指导性,对非小细胞肺癌患者进行化疗时,应尽量个体化用药并选择联合用药方案。Objective：To study chemosensitivity of anticancer drugs in the peripheral blood karyocytes（lymphocytes and granulocytes） of non-small-cell lung cancer（NSCLC） patients,and to choose better chemotherapeutics or combined chemotherapy drugs.Methods： The sensitivity or resistance of 4 kinds of commonly used chemotherapeutic drugs and combined chemotherapy in peripheral blood lymphocytes and granulocytes of 32 cases with non-small-cell lung cancer in vitro by classic MTT colorimetric assay.Results： The individual chemosensitivity of NSCLC were comparatively different.The chemosensitivity of combined drugs was significant superior to single drugs,P〈0.05.Conclusion：The tumour chemosensitivity test in vitro gave some prediction and guidances for the chemotherapy,and it could discover the drug resisting cases.The combined chemotherapy should be selected for NSCLC.

关 键 词：非小细胞肺癌 化疗敏感性 MTT吸光度法 外周血有核细胞 

分 类 号：R734.2[医药卫生—肿瘤]