包含氧化还原酶的WW结构域基因甲基化与食管鳞状细胞癌的关系  

Promoter methylation of WWOX gene in esophageal squamous cell carcinoma

作　　者：董玉然[1] 王益民[2] 郭炜[1] 刘盼[1] 郭艳丽[1] 杨植彬[1] 董稚明[1] 

机构地区：[1]河北医科大学第四医院省肿瘤研究所病理研究室,河北石家庄050011 [2]河北省秦皇岛市第一医院普外科,河北秦皇岛066000

出　　处：《癌变．畸变．突变》2010年第2期91-94,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河北省强势特色学科基金资助项目([2005]52号)

摘　　要：目的:研究食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)包含氧化还原酶的WW结构域(WW domain containing oxidoreductase,WWOX)基因启动子甲基化及mRNA的表达与ESCC发生发展的关系。方法:采用改进的甲基化特异性PCR(methylation specific PCR,MSP)和RT-PCR技术检测69例ESCC组织及48例相应癌旁正常组织中WWOX基因的DNA甲基化和mRNA表达情况。结果:ESCC组织中WWOX基因启动子的甲基化发生率为26.1%(18/69),显著高于癌旁正常组织4.2%(2/48)(P=0.002);Ⅲ、Ⅳ期食管癌患者中WWOX基因甲基化发生率(43.3%,13/30)显著高于Ⅰ、Ⅱ期(12.8%,5/39)(P=0.004);低分化癌组的甲基化发生率(33.3%,4/12)高于高分化(20.0%,3/15)和中分化组(26.2%,11/42),但其差异均无统计学意义(P>0.05)。ESCC组织中WWOX基因mRNA的相对表达量比相应癌旁正常组织低,差异具有统计学意义(P<0.05)。ESCC组织中WWOXmRNA的相对表达量与临床病理因素无明显相关关系(P>0.05)。结论:WWOX基因启动子甲基化可能与ESCC的形成和进展有关。OBJECTIVE：To detect WW domain containing oxidoreductase gene（WWOX）expression and test abnormal promoter methylation of WWOX gene in esophageal squamous cell carcinoma（ESCC）. METHODS： RT-PCR and methylation- specific PCR （MSP） methods were used to detect WWOX gene expression and WWOX promoter methylation in 69 ESCC tissues and 48 corresponding normal tissues. RESULTS： WWOX gene was methylated in 18 of 69 （26.1%） tumor specimens, which was significantly higher than that in corresponding normal tissues （4.2%,2/48,P=0.002）. Methylation frequencies of stagesⅢandⅣtumor tissues （43.3%） were higher than those of stagesⅠandⅡtumor tissues （12.8%）（P=0.004）. Methylation frequencies of poorly differentiated group （33.3%） were higher than those in moderate-（20%）and poor-moderate differentiated groups （26.2%）, but it did not show significant difference （P0.05）. Expression level of WWOX gene mRNA in esophageal cancer tissues was significantly lower when compared with the corresponding normal tissues. No significant correlation was found between the expression level of WWOX gene mRNA and clinical data （P0.05） in ESCC tissues. CONCLUSION： The aberrant promoter methylation of WWOX gene was related to tumorigenesis and progression of esophageal squamous cell carcinoma.

关 键 词：食管鳞状细胞癌 WWOX 甲基化 

分 类 号：R735.1[医药卫生—肿瘤]